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dc.contributor.authorHelle, Øystein Ivar
dc.contributor.authorDullo, Firehun Tsige
dc.contributor.authorLahrberg, Marcel
dc.contributor.authorTinguely, Jean-Claude
dc.contributor.authorHellesø, Olav Gaute
dc.contributor.authorAhluwalia, Balpreet Singh
dc.date.accessioned2023-09-21T13:06:36Z
dc.date.available2023-09-21T13:06:36Z
dc.date.issued2020-04-11
dc.description.abstractStructured illumination microscopy (SIM) enables live-cell super-resolution imaging of subcellular structures at high speeds. At present, linear SIM uses free-space optics to illuminate the sample with the desired light patterns; however, such arrangements are prone to misalignment and add cost and complexity to the microscope. Here, we present an alternative photonic chip-based two-dimensional SIM approach (cSIM) in which the conventional glass sample slide in a microscope is replaced by a planar photonic chip that importantly both holds and illuminates the specimen. The photonic chip reduces the footprint of the light illumination path of SIM to around 4 × 4 cm<sup>2</sup>. An array of optical waveguides on the chip creates standing wave interference patterns at different angles, which illuminate the sample via evanescent fields. High-refractive-index silicon nitride waveguides allow a 2.3 times enhancement in imaging spatial resolution, exceeding the usual 2 times limit of SIM. In summary, cSIM offers a simple, stable and affordable approach for performing two-dimensional super-resolution imaging over a large field of view.en_US
dc.identifier.citationHelle ØI, Dullo FT, Lahrberg M, Tinguely J, Hellesø OG, Ahluwalia BS. Structured illumination microscopy using a photonic chip. Nature Photonics. 2020;14:431-438en_US
dc.identifier.cristinIDFRIDAID 1848867
dc.identifier.doihttps://doi.org/10.1038/s41566-020-0620-2
dc.identifier.issn1749-4885
dc.identifier.issn1749-4893
dc.identifier.urihttps://hdl.handle.net/10037/31146
dc.language.isoengen_US
dc.publisherNature Publishing Groupen_US
dc.relation.journalNature Photonics
dc.relation.projectIDERC-European Research Council: 336716en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleStructured illumination microscopy using a photonic chipen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Med mindre det står noe annet, er denne innførselens lisens beskrevet som Attribution 4.0 International (CC BY 4.0)