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dc.contributor.authorMtenga, Coletha Mathew
dc.contributor.authorStokstad, Maria
dc.contributor.authorJohansen, Tone Kristin Bjordal
dc.contributor.authorKlevar, Siv
dc.contributor.authorMdgela, Robinson H.
dc.contributor.authorMwamengele, G.L.
dc.contributor.authorMichel, P
dc.contributor.authorEscobar, L.
dc.contributor.authorFretin, D.
dc.contributor.authorGodfroid, Jacques
dc.date.accessioned2015-09-16T07:10:25Z
dc.date.available2015-09-16T07:10:25Z
dc.date.issued2015-07-21
dc.description.abstractBackground: Brucellosis is a disease of worldwide public health and economic importance. Successful control is based on knowledge of epidemiology and strains present in an area. In developing countries, most investigations are based on serological assays. This study aimed at investigating a dairy herd experiencing abortions in order to establish within-herd seroprevalence to Brucella spp., identify, characterize Brucella strains by Multiple Loci Variable Number of Tandem Repeats Analysis (MLVA-VNTR) and investigate possible spillover to other species. Results: The within-herd seroprevalence in cattle (n = 200) was 48 % (95 % CI 41–55), using an indirect ELISA, while the Rose Bengal Test (RBT) yielded lower prevalence (21.5 %; 95 % CI 16–27). Two sheep (n = 35) and one goat (n = 50) were seropositive using ELISA while none of the dogs (n = 6) was positive with the RBT. Three Brucella were isolated from an aborted fetus and associated membranes. Real time PCR (IS711), Bruce-ladder and classical biotyping classified the isolates as B. abortus biovar 3. MLVA-VNTR revealed two different but closely related genotypes. The isolates showed unique profiles, providing the first genotypic data from Tanzania. These genotypes were not related to B. abortus biovar 3 reference strain Tulya originally isolated from a human patient in Uganda in 1958, unlike the genotypes isolated and characterized recently in Kenya. High within-herd prevalence, isolation of the pathogen and abortion confirm that B. abortus is circulating in this herd with cattle as reservoir hosts. A low seroprevalence in sheep and goats suggests a spillover of B. abortus from cattle to small ruminants in the herd. Conclusions: This is the first isolation and characterization of B. abortus biovar 3 from a dairy cow with abortion in Tanzania. The origin of the Tanzanian genotypes remain elusive, although they seem to be related to genotypes found in Europe, Turkey and China but not related to B. abortus biovar 3 reference strain or genotypes from Kenya. Importantly, replacement heifers are commonly sourced from large farms like this to smallholder farmers, which poses risk of spread of bacteria to other herds. B. abortus is a significant zoonotic risk and animal health problem in this production system, therefore further studies on humans is recommended.en_US
dc.identifier.citationBMC Veterinary Research (2015) 11:156en_US
dc.identifier.cristinIDFRIDAID 1261921
dc.identifier.doi10.1186/s12917-015-0476-8
dc.identifier.issn1746-6148
dc.identifier.urihttps://hdl.handle.net/10037/8074
dc.identifier.urnURN:NBN:no-uit_munin_7663
dc.language.isoengen_US
dc.publisherBioMed Centralen_US
dc.rights.accessRightsopenAccess
dc.subjectAbortionen_US
dc.subjectBovineen_US
dc.subjectBiotypingen_US
dc.subjectBrucellaen_US
dc.subjectDogen_US
dc.subjectGenotypingen_US
dc.subjectMLVAen_US
dc.subjectPrevalenceen_US
dc.subjectSmall ruminantsen_US
dc.subjectZoonosisen_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480en_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480en_US
dc.titleFirst isolation, identification, phenotypic and genotypic characterization of Brucella abortus biovar 3 from dairy cattle in Tanzaniaen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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