Cyclic nucleotide dependent ATPase activity in inside out vesicles from human erythrocytes
The transport of cyclic nucleotides out of the cell is energy requiring, dependent on ATP- hydrolysis. The ability to stimulate this ATPase is a hallmark of substrates for ABC-transporters e.g. ABCC5, ABCC4. It is believed that ABCC5 transporter has selective high affinity for cGMP. Previous studies support the idea that ABCC5 contribute to cGMP transport by human erythrocytes. Human erythrocyte membrane possesses cGMP transport system that utilizes ATP for its activity. Present study was conducted to measure, “Cyclic nucleotide dependent ATPase activity in inside out vesicles from human erythrocytes” by an In-house assay method and to compare it with commercially available kit to make cost benefit analysis. The main findings were: 1) The inorganic phosphate standard curves were linear for relevant biological concentrations, observed for both in-house and commercial assay. 2) The membrane protein (i.e. IOV) concentrations raised the inorganic phosphate concentrations linearly. 3) It was possible to distinguish cGMP-stimulated activity from basal ATPase activity in hRBC IOV, even though the difference was small. 4) The two phosphate assays (in-house and commercial) had both advantages and disadvantages, none being superior to the other one.
ForlagUiT Norges arktiske universitet
UiT The Arctic University of Norway
Følgende lisensfil er knyttet til denne innførselen:
Viser innførsler relatert til tittel, forfatter og emneord.
Prognostic Impacts of Angiopoietins in NSCLC Tumor Cells and Stroma : VEGF-A Impact Is Strongly Associated with Ang-2 Andersen, Sigve; Dønnem, Tom; Al-Shibli, Khalid Ibrahim; Al-Saad, Samer; Stenvold, Helge; Busund, Lill-Tove; Bremnes, Roy M. (Journal article; Tidsskriftartikkel; Peer reviewed, 2011)Angiopoietins and their receptor Tie-2 are, in concert with VEGF-A, key mediators in angiogenesis. This study evaluates the prognostic impact of all known human angiopoietins (Ang-1, Ang-2 and Ang-4) and their receptor Tie-2, as well as their relation to the prognostic expression of VEGF-A. 335 unselected stage I-IIIA NSCLC-patients were included and tissue samples of respective tumor cells and ...
Humant papillomavirus : en litteraturstudie om HPV, dets relasjon til cancer og tiltak mot videre spredning av virus Gabrielsen, Endre (Master thesis; Mastergradsoppgave, 2012-06-01)I 1983 oppdaget zur Hausen sammenhengen mellom Humant Papillomavirus (HPV) og livmorhalskreft. På denne tiden visste man ikke at det var HPV som var årsaken til at Helaceller kunne leve in vitro. Ny forskning relaterer HPV til en rekke andre cancertyper. En stor andel anal-, oropharyngeal-, penis-, vaginal-, og vulvacancer skyldes HPV. Det er også påvist HPV i tumorvev fra øsofagus, larynx, lunge, ...