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dc.contributor.authorDiekmann, Robin
dc.contributor.authorWolfson, Deanna
dc.contributor.authorSpahn, Christoph
dc.contributor.authorHeilemann, Mike
dc.contributor.authorSchuttpelz, Mark
dc.contributor.authorHuser, Thomas
dc.date.accessioned2017-03-15T12:27:07Z
dc.date.available2017-03-15T12:27:07Z
dc.date.issued2016-12-13
dc.description.abstractImaging non-adherent cells by super-resolution far-field fluorescence microscopy is currently not possible because of their rapid movement while in suspension. Holographic optical tweezers (HOTs) enable the ability to freely control the number and position of optical traps, thus facilitating the unrestricted manipulation of cells in a volume around the focal plane. Here we show that immobilizing non-adherent cells by optical tweezers is sufficient to achieve optical resolution well below the diffraction limit using localization microscopy. Individual cells can be oriented arbitrarily but preferably either horizontally or vertically relative to the microscope’s image plane, enabling access to sample sections that are impossible to achieve with conventional sample preparation and immobilization. This opens up new opportunities to super-resolve the nanoscale organization of chromosomal DNA in individual bacterial cells.en_US
dc.descriptionPublished version. Source at <a href=http://dx.doi.org/10.1038/ncomms13711> http://dx.doi.org/10.1038/ncomms13711 </a>en_US
dc.identifier.citationDiekmann R. et.al.: Nanoscopy of bacterial cells immobilized by holographic optical tweezers. Nature Communications. 2016;7en_US
dc.identifier.cristinIDFRIDAID 1431844
dc.identifier.doi10.1038/ncomms13711
dc.identifier.issn2041-1723
dc.identifier.urihttps://hdl.handle.net/10037/10698
dc.language.isoengen_US
dc.publisherNature Publishing Groupen_US
dc.relation.journalNature Communications
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473en_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Molecular biology: 473en_US
dc.titleNanoscopy of bacterial cells immobilized by holographic optical tweezersen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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