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dc.contributor.authorAhluwalia, Balpreet Singh
dc.contributor.authorCoucheron, David Andre
dc.contributor.authorHelle, Øystein Ivar
dc.contributor.authorØie, Cristina Ionica
dc.contributor.authorDullo, Firehun Tsige
dc.date.accessioned2018-01-22T09:14:44Z
dc.date.available2018-01-22T09:14:44Z
dc.date.issued2017
dc.description.abstractSuper-resolution optical microscopy, commonly referred to as optical nanoscopy, has enabled imaging of biological samples with a resolution that was only achievable previously using electron microscopy. Optical nanoscopy is a rapidly growing field, with several different techniques and implementations that overcome the diffraction limit of light. However, the common nanoscope continues to be a rather complex, expensive and bulky instrument. Direct stochastic optical reconstruction microscopy (dSTORM) imaging was recently demonstrated using a waveguide platform for excitation in combination with a simple microscope for imaging. High refractive index waveguide materials have a high intensity evanescent field stretching around 100-200 nm outside the guiding material, which is ideally suited for total internal reflection fluorescence (TIRF) excitation over large areas. We demonstrate dSTORM imaging of the plasma membrane of liver sinusoidal endothelial cells (LSECs) and trophoblasts (HTR-8 cells) using waveguide excitation, with resolution down to around 70 nm. Additionally, we present TIRF imaging of LSEC micro-tubules over a 500 μm x 500 μm area, laying the foundation for large field of view (f-o-v) nanoscopy.en_US
dc.description.sponsorshipThe research leading to these results has received funding from the European Union's Seventh Framework Programme (FP7/2007-2013) under grant agreement n° 336716 (to B.S.A.). The work was also supported by UiT The Arctic University of Norway (Tematiske Satsinger to B.S.A.).en_US
dc.identifier.citationAhluwalia BS, Coucheron DA, Helle ØI, Øie CI, Dullo FT. Chip Based Nanoscopy: Towards Integration and High-throughput Imaging. Proceedings of SPIE, the International Society for Optical Engineering. 2017;10350en_US
dc.identifier.cristinIDFRIDAID 1516254
dc.identifier.doi10.1117/12.2273902
dc.identifier.issn0277-786X
dc.identifier.issn1996-756X
dc.identifier.urihttps://hdl.handle.net/10037/11997
dc.language.isoengen_US
dc.publisherSPIEen_US
dc.relation.ispartofCoucheron, D.A. (2021). Waveguide-based Excitation for High-throughput Imaging. (Doctoral thesis). <a href=https://hdl.handle.net/10037/20695>https://hdl.handle.net/10037/20695</a>
dc.relation.journalProceedings of SPIE, the International Society for Optical Engineering
dc.relation.projectIDUniversitetet i Tromsø: Tematiske Satsingeren_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/336716////en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectwaveguidesen_US
dc.subjectmicroscopyen_US
dc.subjectobjectivesen_US
dc.subjectdiffractionen_US
dc.subjectluminescenceen_US
dc.subjectsuper resolutionen_US
dc.subjectsuper resolution microscopyen_US
dc.subjectoptical microscopyen_US
dc.subjectplasmaen_US
dc.subjectmicroscopesen_US
dc.titleChip Based Nanoscopy: Towards Integration and High-throughput Imagingen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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