Show simple item record

dc.contributor.authorGuljamow, Arthur
dc.contributor.authorKreische, Marco
dc.contributor.authorIshida, Keishi
dc.contributor.authorLiaimer, Anton
dc.contributor.authorAltermark, Bjørn
dc.contributor.authorBähr, Lars
dc.contributor.authorHertweck, Christian
dc.contributor.authorDittmann, Elke
dc.date.accessioned2018-07-27T12:40:14Z
dc.date.available2018-07-27T12:40:14Z
dc.date.issued2017-09-22
dc.description.abstractTerrestrial symbiotic cyanobacteria of the genus <i>Nostoc</i> exhibit a large potential for the production of bioactive natural products of the nonribosomal peptide, polyketide, and ribosomal peptide classes, and yet most of the biosynthetic gene clusters are silent under conventional cultivation conditions. In the present study, we utilized a high-density cultivation approach recently developed for phototrophic bacteria to rapidly generate biomass of the filamentous bacteria up to a density of 400 g (wet weight)/liter. Unexpectedly, integrated transcriptional and metabolomics studies uncovered a major reprogramming of the secondary metabolome of two <i>Nostoc</i> strains at high culture density and a governing effect of extracellular signals in this process. The holistic approach enabled capturing and structural elucidation of novel variants of anabaenopeptin, including one congener with potent allelopathic activity against a strain isolated from the same habitat. The study provides a snapshot on the role of cell-type-specific expression for the formation of natural products in cyanobacteria.en_US
dc.description.sponsorshipThe German Research Foundation DFG-funded Collaborative Research Centre ChemBioSysen_US
dc.descriptionSource at <a href=https://doi.org/10.1128/AEM.01510-17> https://doi.org/10.1128/AEM.01510-17</a>.en_US
dc.identifier.citationGuljamow, A., Kreische, M., Ishida, K., Liaimer, A., Altermark, B., Bähr, L., ... Dittmann, E. (2017). High-density cultivation of terrestrial Nostoc strains leads to reprogramming of secondary metabolome. Applied and Environmental Microbiology, 83(23:e01510-17), 1-15. https://doi.org/10.1128/AEM.01510-17en_US
dc.identifier.cristinIDFRIDAID 1497865
dc.identifier.doi10.1128/AEM.01510-17
dc.identifier.issn0099-2240
dc.identifier.issn1098-5336
dc.identifier.urihttps://hdl.handle.net/10037/13297
dc.language.isoengen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.journalApplied and Environmental Microbiology
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497en_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497en_US
dc.subjectallelopathyen_US
dc.subjectcellular differentiationen_US
dc.subjectcyanobacteriaen_US
dc.subjectnatural productsen_US
dc.subjectnonribosomal peptideen_US
dc.titleHigh-density cultivation of terrestrial Nostoc strains leads to reprogramming of secondary metabolomeen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record