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dc.contributor.authorNguyen, Nga
dc.contributor.authorSuokas, Marko
dc.contributor.authorKarppinen, Katja
dc.contributor.authorVuosku, Jaana
dc.contributor.authorJaakola, Laura
dc.contributor.authorHäggman, Hely
dc.date.accessioned2018-09-17T07:53:18Z
dc.date.available2018-09-17T07:53:18Z
dc.date.issued2018-07-02
dc.description.abstractBilberry (<i>Vaccinium myrtillus</i> L.) fruits are an excellent natural resource for human diet because of their special flavor, taste and nutritional value as well as medical properties. Bilberries are recognized for their high anthocyanin content and many of the genes involved in the anthocyanin biosynthesis have been characterized. So far, neither genomic nor RNA-seq data have been available for the species. In the present study, we de novo sequenced two bilberry fruit developmental stages, unripe green (G) and ripening (R). A total of 57,919 unigenes were assembled of which 80.2% were annotated against six public protein databases. The transcriptome served as exploratory data to identify putative transcription factors related to fruit ripening. Differentially expressed genes (DEGs) between G and R stages were prominently upregulated in R stage with the functional annotation indicating their main roles in active metabolism and catalysis. The unigenes encoding putative ripening-related regulatory genes, including members of NAC, WRKY, LOB, ERF, ARF and ABI families, were analysed by qRT-PCR at five bilberry developmental stages. Our <i>de novo</i> transcriptome database contributes to the understanding of the regulatory network associated with the fruit ripening in bilberry and provides the first dataset for wild Vaccinium species acquired by NGS technology.en_US
dc.description.sponsorshipCIMO Tauno Tönning Foundation Interreg Nord Regional Council of Laplanden_US
dc.descriptionSource at <a href=https://doi.org/10.1038/s41598-018-28158-7> https://doi.org/10.1038/s41598-018-28158-7</a>.en_US
dc.identifier.citationNguyen, N., Suokas, M., Karppinen, K., Vuosku, J., Jaakola, L. & Häggman, H. (2018). Recognition of candidate transcription factors related to bilberry fruit ripening by de novo transcriptome and qRT-PCR analyses. Scientific Reports, 8(9943). https://doi.org/10.1038/s41598-018-28158-7en_US
dc.identifier.cristinIDFRIDAID 1599733
dc.identifier.doi10.1038/s41598-018-28158-7
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/10037/13807
dc.language.isoengen_US
dc.publisherNature Publishing Groupen_US
dc.relation.journalScientific Reports
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480en_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480en_US
dc.titleRecognition of candidate transcription factors related to bilberry fruit ripening by de novo transcriptome and qRT-PCR analysesen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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