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dc.contributor.authorKarppinen, Katja Hannele
dc.contributor.authorTegelberg, Pinja
dc.contributor.authorHäggman, Hely
dc.contributor.authorJaakola, Laura
dc.date.accessioned2018-11-08T14:13:37Z
dc.date.available2018-11-08T14:13:37Z
dc.date.issued2018-08-29
dc.description.abstractRipening of non-climacteric bilberry (Vaccinium myrtillus L.) fruit is characterized by a high accumulation of health-beneficial anthocyanins. Plant hormone abscisic acid (ABA) and sucrose have been shown to be among the central signaling molecules coordinating non-climacteric fruit ripening and anthocyanin accumulation in some fruits such as strawberry. Our earlier studies have demonstrated an elevation in endogenous ABA level in bilberry fruit at the onset of ripening indicating a role for ABA in the regulation of bilberry fruit ripening. In the present study, we show that the treatment of unripe green bilberry fruits with exogenous ABA significantly promotes anthocyanin biosynthesis and accumulation both in fruits attached and detached to the plant. In addition, ABA biosynthesis inhibitor, fluridone, delayed anthocyanin accumulation in bilberries. Exogenous ABA also induced the expression of several genes involved in cell wall modification in ripening bilberry fruits. Furthermore, silencing of VmNCED1, the key gene in ABA biosynthesis, was accompanied by the down-regulation in the expression of key anthocyanin biosynthetic genes. In contrast, the treatment of unripe green bilberry fruits with exogenous sucrose or glucose did not lead to an enhancement in the anthocyanin accumulation neither in fruits attached to plant nor in post-harvest fruits. Moreover, sugars failed to induce the expression of genes associated in anthocyanin biosynthesis or ABA biosynthesis while could elevate expression of some genes associated with cell wall modification in post-harvest bilberry fruits. Our results demonstrate that ABA plays a major role in the regulation of ripening-related processes such as anthocyanin biosynthesis and cell wall modification in bilberry fruit, whereas sugars seem to have minor regulatory roles in the processes. The results indicate that the regulation of bilberry fruit ripening differs from strawberry that is currently considered as a model of nonclimacteric fruit ripening. In this study, we also identified transcription factors, which expression was enhanced by ABA, as potential regulators of ABA-mediated bilberry fruit ripening processes.en_US
dc.description.sponsorshipThe Finnish Cultural Foundation: ? Niemi Foundation: ? Otto A. Malm Foundation: ? Osk. Huttunen Foundation: ? Interreg Nord: ?en_US
dc.descriptionSource at: <a href=http://doi.org/10.3389/fpls.2018.01259> http://doi.org/10.3389/fpls.2018.01259</a>en_US
dc.identifier.citationKarppinen, K., Tegelberg, P., Häggman, H. & Jaakola, L. (2018). Abscisic acid regulates anthocyanin biosynthesis and gene expression associated with cell wall modification in ripening bilberry (vaccinium myrtillus l.) fruits. <i>Frontiers in Plant Science, 9</i>(1259). http://doi.org/10.3389/fpls.2018.01259en_US
dc.identifier.cristinIDFRIDAID 1622754
dc.identifier.doi10.3389/fpls.2018.01259
dc.identifier.issn1664-462X
dc.identifier.urihttps://hdl.handle.net/10037/14138
dc.language.isoengen_US
dc.publisherFrontiers Mediaen_US
dc.relation.journalFrontiers in Plant Science
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480en_US
dc.titleAbscisic acid regulates anthocyanin biosynthesis and gene expression associated with cell wall modification in ripening bilberry (vaccinium myrtillus l.) fruitsen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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