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dc.contributor.authorAhmad, Azeem
dc.contributor.authorDubey, Vishesh
dc.contributor.authorSingh, Vijay
dc.contributor.authorTinguely, Jean-Claude
dc.contributor.authorØie, Cristina Ionica
dc.contributor.authorWolfson, Deanna
dc.contributor.authorMehta, Dalip Singh
dc.contributor.authorSo, Peter T. C.
dc.contributor.authorAhluwalia, Balpreet Singh
dc.date.accessioned2019-02-12T09:54:18Z
dc.date.available2019-02-12T09:54:18Z
dc.date.issued2018-08-22
dc.description.abstractRed blood cells (RBCs) have the ability to undergo morphological deformations during microcirculation, such as changes in surface area, volume and sphericity. Optical waveguide trapping is suitable for trapping, propelling and deforming large cell populations along the length of the waveguide. Bright field microscopy employed with waveguide trapping does not provide quantitative information about structural changes. Here, we have combined quantitative phase microscopy and waveguide trapping techniques to study changes in RBC morphology during planar trapping and transportation. By using interference microscopy, time-lapsed interferometric images of trapped RBCs were recorded in real-time and subsequently utilized to reconstruct optical phase maps. Quantification of the phase differences before and after trapping enabled study of the mechanical effects during planar trapping. During planar trapping, a decrease in the maximum phase values, an increase in the surface area and a decrease in the volume and sphericity of RBCs were observed. QPM was used to analyze the phase values for two specific regions within RBCs: the annular rim and the central donut. The phase value of the annular rim decreases whereas it increases for the central donut during planar trapping. These changes correspond to a redistribution of cytosol inside the RBC during planar trapping and transportation.en_US
dc.description.sponsorshipThe European Research Council (project number 336716) The Norwegian Centre for International Cooperation in Education, SIU-Norway The University Grant Commission (UGC) India BioSym IRG in Singapore-MIT Science & Research Center The National Institutes of Healthen_US
dc.descriptionSource at <a href=https://doi.org/10.1039/c8lc00356d> https://doi.org/10.1039/c8lc00356d</a>.en_US
dc.identifier.citationAhmad, A., Dubey, V., Singh, V.R., Tinguely, J.-C., Øie, C.I., Wolfson, D.L., ... Ahluwalia, B.S. (2018). Quantitative phase microscopy of red blood cells during planar trapping and propulsion. <i>Lab on a Chip, 18</i>(19), 3025-3036. https://doi.org/10.1039/c8lc00356den_US
dc.identifier.cristinIDFRIDAID 1613567
dc.identifier.doi10.1039/c8lc00356d
dc.identifier.issn1473-0189
dc.identifier.urihttps://hdl.handle.net/10037/14675
dc.language.isoengen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.journalLab on a Chip
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7-IDEAS-ERC/336716/EU/High-speed chip-based nanoscopy to discover real-time sub-cellular dynamics/NANOSCOPY/en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Mathematics and natural science: 400::Physics: 430en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Fysikk: 430en_US
dc.titleQuantitative phase microscopy of red blood cells during planar trapping and propulsionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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