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dc.contributor.authorStriberny, Anja
dc.contributor.authorJørgensen, Even Hjalmar
dc.contributor.authorKlopp, Christophe
dc.contributor.authorMagnanou, Elodie
dc.date.accessioned2019-08-09T06:57:44Z
dc.date.available2019-08-09T06:57:44Z
dc.date.issued2019-06-27
dc.description.abstract<i>Background</i>: The Arctic charr (<i>Salvelinus alpinus</i>) has a highly seasonal feeding cycle that comprises long periods of voluntary fasting and a short but intense feeding period during summer. Therefore, the charr represents an interesting species for studying appetite-regulating mechanisms in fish. <p> <p><i>Results</i>: In this study, we compared the brain transcriptomes of fed and feed deprived charr over a 4 weeks trial during their summer feeding season. Despite prominent differences in body condition between fed and feed deprived charr at the end of the trial, feed deprivation affected the brain transcriptome only slightly. In contrast, the transcriptome differed markedly over time in both fed and feed deprived charr, indicating strong shifts in basic cell metabolic processes possibly due to season, growth, temperature, or combinations thereof. The GO enrichment analysis revealed that many biological processes appeared to change in the same direction in both fed and feed deprived fish. In the feed deprived charr processes linked to oxygen transport and apoptosis were down- and up-regulated, respectively. Known genes encoding for appetite regulators did not respond to feed deprivation. Gene expression of <i>Deiodinase 2 (DIO2)</i>, an enzyme implicated in the regulation of seasonal processes in mammals, was lower in response to season and feed deprivation. We further found a higher expression of <i>VGF</i> (non-acronymic) in the feed deprived than in the fed fish. This gene encodes for a neuropeptide associated with the control of energy metabolism in mammals, and has not been studied in relation to regulation of appetite and energy homeostasis in fish.<p> <p><i>Conclusions</i>: In the Arctic charr, external and endogenous seasonal factors for example the increase in temperature and their circannual growth cycle, respectively, evoke much stronger responses in the brain than 4 weeks feed deprivation. The absence of a central hunger response in feed deprived charr give support for a strong resilience to the lack of food in this high Arctic species. DIO2 and VGF may play a role in the regulation of energy homeostasis and need to be further studied in seasonal fish.en_US
dc.description.sponsorshipUiT - The Arctic University of Tromsø French Ministries of Europe and Foreign affairs Ministère de l’Enseignement supérieur, de la Recherche et de l’Innovation – MESRI The Aurora mobility programmeen_US
dc.descriptionSource at <a href=https://doi.org/10.1186/s12864-019-5874-z>https://doi.org/10.1186/s12864-019-5874-z. </a> © The Author(s). 2019en_US
dc.identifier.citationStriberny, A., Jørgensen, E.H., Klopp, C. & Magnanou, E. (2019). Arctic charr brain transcriptome strongly affected by summer seasonal growth but only subtly by feed deprivation. <i>BMC Genomics, 20</i>, 529. https://doi.org/10.1186/s12864-019-5874-zen_US
dc.identifier.cristinIDFRIDAID 1707563
dc.identifier.doi10.1186/s12864-019-5874-z
dc.identifier.issn1471-2164
dc.identifier.urihttps://hdl.handle.net/10037/15880
dc.language.isoengen_US
dc.publisherBMCen_US
dc.relation.journalBMC Genomics
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920en_US
dc.subjectVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920en_US
dc.subjectFeed deprivationen_US
dc.subjectSalvelinus alpinusen_US
dc.subjectRNA-seqen_US
dc.subjectBrain transcriptomeen_US
dc.subjectNeuropeptidesen_US
dc.subjectSeasonen_US
dc.titleArctic charr brain transcriptome strongly affected by summer seasonal growth but only subtly by feed deprivationen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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