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dc.contributor.authorStröhl, Florian
dc.contributor.authorOpstad, Ida Sundvor
dc.contributor.authorTinguely, Jean-Claude
dc.contributor.authorDullo, Firehun Tsige
dc.contributor.authorMela, Ioanna
dc.contributor.authorOsterrieth, Johannes W.M
dc.contributor.authorAhluwalia, Balpreet Singh
dc.contributor.authorKaminski, Clemens F.
dc.date.accessioned2019-09-02T14:22:38Z
dc.date.available2019-09-02T14:22:38Z
dc.date.issued2019-08-22
dc.description.abstractLabelfree nanoscopy encompasses optical imaging with resolution in the 100 nm range using visible wavelengths. Here, we present a labelfree nanoscopy method that combines coherent imaging techniques with waveguide microscopy to realize a <i>super-condenser</i> featuring maximally inclined coherent darkfield illumination with artificially stretched wave vectors due to large refractive indices of the employed Si<sub>3</sub>N<sub>4</sub> waveguide material. We produce the required coherent plane wave illumination for Fourier ptychography over imaging areas 400 μm2 in size via adiabatically tapered single-mode waveguides and tackle the overlap constraints of the Fourier ptychography phase retrieval algorithm two-fold: firstly, the directionality of the illumination wave vector is changed sequentially via a multiplexed input structure of the waveguide chip layout and secondly, the wave vector modulus is shortend via step-wise increases of the illumination light wavelength over the visible spectrum. We test the method in simulations and in experiments and provide details on the underlying image formation theory as well as the reconstruction algorithm. While the generated Fourier ptychography reconstructions are found to be prone to image artefacts, an alternative coherent imaging method, rotating coherent scattering microscopy (ROCS), is found to be more robust against artefacts but with less achievable resolution.en_US
dc.description.sponsorshipEuropean Molecular Biology Organisation Marie Skłodowska-Curie actions European Research Council Physical Sciences Research Council Medical Research Council Wellcome Trust Infinitus China Ltd.en_US
dc.descriptionSource at <a href=https://doi.org/10.1364/OE.27.025280>https://doi.org/10.1364/OE.27.025280. </a>en_US
dc.identifier.citationStröhl, F., Opstad, I.S., Tinguely, J-C., Dullo, F.T., Mela, I., Osterrieth, J.W. ... Kaminski, C.F. (2019). Super-condenser enables labelfree nanoscopy. <i>Optics Express, 27</i>(18), 25280-25292. https://doi.org/10.1364/OE.27.025280en_US
dc.identifier.cristinIDFRIDAID 1718199
dc.identifier.doi10.1364/OE.27.025280
dc.identifier.issn1094-4087
dc.identifier.urihttps://hdl.handle.net/10037/16064
dc.language.isoengen_US
dc.publisherThe Optical Societyen_US
dc.relation.journalOptics Express
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/336716/EU/High-speed chip-based nanoscopy to discover real-time sub-cellular dynamics/NANOSCOPYen_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Mathematics and natural science: 400::Physics: 430en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Fysikk: 430en_US
dc.titleSuper-condenser enables labelfree nanoscopyen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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