Adaptive fluctuation imaging captures rapid subcellular dynamics

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https://hdl.handle.net/10037/17829
DOI
https://doi.org/10.1117/12.2526846
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Date
2019-07-22
Type
Journal article
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Peer reviewed

Author
Opstad, Ida Sundvor; Ströhl, Florian; Birgisdottir, Åsa birna; Maldonado, Sebastián Andrés Acuña; Kalstad, Trine; Myrmel, Truls; Agarwal, Krishna; Ahluwalia, Balpreet Singh
Abstract
In this work we have explored the live-cell friendly nanoscopy method Multiple Signal Classification Algorithm (MUSICAL) for multi-colour imaging of various organelles and sub-cellular structures in the cardiomyoblast cell line H2c9. We have tested MUSICAL for fast (up to 230Hz), multi-colour time-lapse sequences of various sub-cellular structures (mitochondria, endoplasmic reticulum, microtubules, endosomes and nuclei) in living cells using low excitation-light dose. Challenges and opportunities with applying MUSICAL for studying rapid sub-cellular dynamics are discussed.
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Copyright 2019 Society of Photo‑Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this publication for a fee or for commercial purposes, and modification of the contents of the publication are prohibited.
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Society of Photo-optical Instrumentation Engineers (SPIE)
Citation
Opstad IS, Ströhl F, Birgisdottir Åb, Maldonado SAA, Kalstad T, Myrmel T, Agarwal K, Ahluwalia BS. Adaptive fluctuation imaging captures rapid subcellular dynamics. Proceedings of SPIE, the International Society for Optical Engineering. 2019;11076
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