Direct and Indirect Effect via Endophytism of Entomopathogenic Fungi on the Fitness of Myzus persicae and Its Ability to Spread PLRV on Tobacco

Abstract

Aphids are major crop pests that are feeding on plant sap and transmitting plant viruses, thus inducing high yield losses worldwide. As chemical pesticides are decreasingly used in plant protection, fungi that cause disease to insects (entomopathogenic fungi) are one of the promising alternatives. They are commonly applied by spraying plants to protect them against herbivores. When applied, some fungi penetrate and live within plant tissues, thus helping to internally protect from insect attacks and other plant diseases. The aim of our study was to assess the effects of entomopathogenic fungi (EPF) applied firstly by contact after insect direct spraying, secondly by endophytic plant inoculation, and thirdly by associated both methods assessing the green peach aphid performances. The impact of the presence of endophytic entomopathogenic fungi (EEPF) in plant tissues on virus transmission by aphids was also considered. We found that the EPF <i>Beauveria bassiana</i> killed the green peach aphid and reduced its fecundity regardless of the application method. On fungal-inoculated plants, there was also a high mortality of aphid nymphs and infection by the potato leafroll virus (PLRV) was delayed by about a week with the EEPF treatment compared to fungal-free plants. This study showed that spraying plant leaves with EPF not only has a direct insecticidal effect against insects but could also have beneficial side effects for the plant against viruses.