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dc.contributor.authorHupało, Kamil
dc.contributor.authorMajaneva, Markus
dc.contributor.authorCzachur, Molly Victoria
dc.contributor.authorSire, Lucas
dc.contributor.authorMarquina, Daniel
dc.contributor.authorLijtmaer, Darío A.
dc.contributor.authorIvanov, Vladislav
dc.contributor.authorLeidenberger, Sonja
dc.contributor.authorČiampor, Fedor
dc.contributor.authorČiamporová-Zat'ovičová, Zuzana
dc.contributor.authorMendes, Izabela S.
dc.contributor.authorDesiderato, Andrea
dc.contributor.authorTopstad, Lasse
dc.contributor.authorMeganck, Kenny
dc.contributor.authorHariz Z. A., Danial
dc.contributor.authorKjærstad, Gaute
dc.contributor.authorLin, Xiao-Long
dc.contributor.authorPrice, Benjamin
dc.contributor.authorStevens, Mark
dc.contributor.authorEkrem, Torbjørn
dc.contributor.authorDeiner, Kristy
dc.date.accessioned2021-05-26T07:03:40Z
dc.date.available2021-05-26T07:03:40Z
dc.date.issued2020-10-24
dc.description.abstractAs global biodiversity declines, there is an increasing need to create an educated and engaged society. Having people of all ages participate in measuring biodiversity where they live helps to create awareness. Recently, the use of environmental DNA (eDNA) for biodiversity surveys has gained momentum. Here, we explore whether sampling eDNA and sequencing it can be used as a means of rapidly surveying urban biodiversity for educational purposes. We sampled 2 × 1 L of water from each of 15 locations in the city of Trondheim, Norway, including a variety of freshwater, marine, and brackish habitats. DNA was extracted, amplified in triplicate targeting the barcoding fragment of COI gene, and sequenced. The obtained data were analyzed on the novel mBRAVE platform, an online open‐access software and computing resource. The water samples were collected in 2 days by two people, and the laboratory analysis was completed in 5 days by one person. Overall, we detected the presence of 506 BINs identified as belonging to 435 taxa, representing at least 265 putative species. On average, only 5.4% of the taxa were shared among six replicates per site. Based on the observed diversity, three distinct clusters were detected and related to the geographic distribution of sites. There were some taxa shared between the habitats, with a substantial presence of terrestrial biota. Here we propose a new form of BioBlitz, where with noninvasive sampling effort combined with swift processing and straightforward online analyses, hundreds of species can be detected. Thus, using eDNA analysis of water is useful for rapid biodiversity surveys and valuable for educational purposes. We show that rapid eDNA surveys, combined with openly available services and software, can be used as an educational tool to raise awareness about the importance of biodiversity.en_US
dc.identifier.citationHupało, Majaneva M, Czachur, Sire, Marquina, Lijtmaer, Ivanov, Leidenberger, Čiampor F, Čiamporová-Zat'ovičová, Mendes, Desiderato, Topstad L, Meganck, Hariz Z. A, Kjærstad G, Lin XL, Price, Stevens M, Ekrem T, Deiner K. An urban Blitz with a twist: rapid biodiversity assessment using aquatic environmental DNA. Environmental DNA. 2020en_US
dc.identifier.cristinIDFRIDAID 1873810
dc.identifier.doi10.1002/edn3.152
dc.identifier.issn2637-4943
dc.identifier.urihttps://hdl.handle.net/10037/21245
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalEnvironmental DNA
dc.relation.urihttps://onlinelibrary.wiley.com/doi/10.1002/edn3.152
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Mathematics and natural science: 400en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400en_US
dc.titleAn urban Blitz with a twist: rapid biodiversity assessment using aquatic environmental DNAen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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