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dc.contributor.advisorHafstad, Anne
dc.contributor.advisorLund, Trine
dc.contributor.authorBryn, Cathrine Furuheim
dc.date.accessioned2021-10-28T13:02:41Z
dc.date.available2021-10-28T13:02:41Z
dc.date.issued2021-05-30
dc.description.abstractHeart failure is a condition with increasing incidence, and remains a global pandemic. In vitro studies of molecular mechanisms aid in understanding the processes leading to and augmenting heart failure. The H9c2 cell line is an established model for the research of cardiac metabolism, toxicity studies and heart failure, mainly due to its origin from myocardial tissue. It possesses the ability to differentiate from an embryonic undifferentiated phenotype toward a cardiac muscular phenotype, induced by all-trans-retinoic acid (RA) and low serum concentrations in the culturing media. The alterations following differentiation increases its resemblance with adult cardiomyocytes with regard to morphology and metabolism. This study aims to further increase knowledge about the cell line’s metabolic and morphologic characteristics after RA-treatment. We differentiated H9c2 cells for 5, 10 and 15 days and assessed the cells by respirometry, real-time qPCR and immunostaining. Our results are not conclusive, but still shows major remodeling during RA-induced maturing of the H9c2 cells. During RA-induced differentiation the cells appear larger and elongated, ordering themselves in a parallel fashion. Results from qPCR shows an early increase in expression of structural genes, while metabolic genes are upregulated later in the differentiation. Results from respirometry shows increased oxidative phosphorylation and increased spare capacity due to increased mitochondrial content.en_US
dc.identifier.urihttps://hdl.handle.net/10037/22870
dc.language.isoengen_US
dc.publisherUiT Norges arktiske universiteten_US
dc.publisherUiT The Arctic University of Norwayen_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)
dc.subject.courseIDMED-3950
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750en_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750en_US
dc.titleMetabolic, morphologic and translational alterations during differentiation of the H9c2 cardiomyoblast cell lineen_US
dc.typeMaster thesisen_US
dc.typeMastergradsoppgaveen_US


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