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Mitochondrial dynamics and quantification of mitochondria-derived vesicles in cardiomyoblasts using structured illumination microscopy

Permanent lenke
https://hdl.handle.net/10037/23283
DOI
https://doi.org/10.1002/jbio.202100305
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article.pdf (2.487Mb)
Publisert versjon (PDF)
Dato
2021-11-12
Type
Journal article
Tidsskriftartikkel
Peer reviewed

Forfatter
Opstad, Ida S.; Godtliebsen, Gustav; Ahluwalia, Balpreet Singh; Myrmel, Truls; Agarwal, Krishna; Birgisdottir, Åsa Birna
Sammendrag
Mitochondria are essential energy-providing organelles of particular importance in energy-demanding tissue such as the heart. The production of mitochondria-derived vesicles (MDVs) is a cellular mechanism by which cells ensure a healthy pool of mitochondria. These vesicles are small and fast-moving objects not easily captured by imaging. In this work, we have tested the ability of the optical super-resolution technique 3DSIM to capture high-resolution images of MDVs. We optimized the imaging conditions both for high-speed video microscopy and fixed-cell imaging and analysis. From the 3DSIM videos, we observed an abundance of MDVs and many dynamic mitochondrial tubules. The density of MDVs in cells was compared for cells under normal growth conditions and cells during metabolic perturbation. Our results indicate a higher abundance of MDVs in H9c2 cells during glucose deprivation compared with cells under normal growth conditions. Furthermore, the results reveal a large untapped potential of 3DSIM in MDV research.
Er en del av
Godtliebsen, G. (2024). Exploring mitochondrial quality control mechanisms and mitochondria-lipid droplet interactions in cardiac cell models. (Doctoral thesis). https://hdl.handle.net/10037/32271
Forlag
Wiley
Sitering
Opstad, Godtliebsen, Ahluwalia, Myrmel, Agarwal, Birgisdottir. Mitochondrial dynamics and quantification of mitochondria-derived vesicles in cardiomyoblasts using structured illumination microscopy. Journal of Biophotonics. 2021
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  • Artikler, rapporter og annet (UB) [3245]
Copyright 2021 The Author(s)

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