Mitochondrial dynamics and quantification of mitochondria-derived vesicles in cardiomyoblasts using structured illumination microscopy
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https://hdl.handle.net/10037/23283Date
2021-11-12Type
Journal articleTidsskriftartikkel
Peer reviewed
Author
Opstad, Ida S.; Godtliebsen, Gustav; Ahluwalia, Balpreet Singh; Myrmel, Truls; Agarwal, Krishna; Birgisdottir, Åsa BirnaAbstract
Mitochondria are essential energy-providing organelles of particular importance in energy-demanding tissue such as the heart. The production of mitochondria-derived vesicles (MDVs) is a cellular mechanism by which cells ensure a healthy pool of mitochondria. These vesicles are small and fast-moving objects not easily captured by imaging. In this work, we have tested the ability of the optical super-resolution technique 3DSIM to capture high-resolution images of MDVs. We optimized the imaging conditions both for high-speed video microscopy and fixed-cell imaging and analysis. From the 3DSIM videos, we observed an abundance of MDVs and many dynamic mitochondrial tubules. The density of MDVs in cells was compared for cells under normal growth conditions and cells during metabolic perturbation. Our results indicate a higher abundance of MDVs in H9c2 cells during glucose deprivation compared with cells under normal growth conditions. Furthermore, the results reveal a large untapped potential of 3DSIM in MDV research.
Is part of
Godtliebsen, G. (2024). Exploring mitochondrial quality control mechanisms and mitochondria-lipid droplet interactions in cardiac cell models. (Doctoral thesis). https://hdl.handle.net/10037/32271Publisher
WileyCitation
Opstad, Godtliebsen, Ahluwalia, Myrmel, Agarwal, Birgisdottir. Mitochondrial dynamics and quantification of mitochondria-derived vesicles in cardiomyoblasts using structured illumination microscopy. Journal of Biophotonics. 2021Metadata
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