Show simple item record

Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay

dc.contributor.authorNilsson, Per
dc.contributor.authorPettersen, Kristin
dc.contributor.authorOppermann, Martin
dc.contributor.authorSkjeflo, Espen Waage
dc.contributor.authorFure, Hilde
dc.contributor.authorChristiansen, Dorte
dc.contributor.authorMollnes, Tom Eirik
dc.date.accessioned2022-03-01T12:44:14Z
dc.date.available2022-03-01T12:44:14Z
dc.date.issued2021-04-14
dc.description.abstractEnzyme-linked immunosorbent assay (ELISA) enables fast and simple quantification of analytes in the pico- to nanogram range in complex samples. Here, we describe an ELISA for the detection of porcine C3a as a marker for complement activation. Antibody specificity is critical for a robust assay. This assay is based on a pair of antibodies specific for the porcine C3a molecule and thus does not react with native C3.en_US
dc.descriptionThis version of the article has been accepted for publication, after peer review and is subject to Springer Nature’s <a href=https://www.springernature.com/gp/open-research/policies/accepted-manuscript-terms>AM terms of use</a>, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at <a href=http://dx.doi.org/10.1007/978-1-0716-1016-9_5>http://dx.doi.org/10.1007/978-1-0716-1016-9_5</a>.en_US
dc.identifier.citationNilsson P, Pettersen K, Oppermann, Skjeflo EW, Fure H, Christiansen D, Mollnes TE. Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay. Methods in molecular biology. 2021;2227:51-59en_US
dc.identifier.cristinIDFRIDAID 1994727
dc.identifier.doi10.1007/978-1-0716-1016-9_5
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.urihttps://hdl.handle.net/10037/24203
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.relation.journalMethods in molecular biology
dc.relation.projectIDNorges forskningsråd: 223255en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 Springer Natureen_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Biochemistry: 476en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Biokjemi: 476en_US
dc.titleQuantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assayen_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail
Name:
article.pdf
Size:
5.279Mb
Format:
PDF
View/Open

This item appears in the following collection(s)

Show simple item record