Getting ready for host invasion: Elevated expression and action of xyloglucan endotransglucosylases/hydrolases in developing haustoria of the holoparasitic angiosperm Cuscuta
Permanent lenke
https://hdl.handle.net/10037/24909Dato
2015-11-11Type
Journal articleTidsskriftartikkel
Peer reviewed
Forfatter
Olsen, Stian; Ketelsen Striberny, Bernd; Hollmann, Julien; Schwacke, Rainer; Popper, Zoe A.; Krause, KirstenSammendrag
Changes in cell walls have been previously observed in the mature infection organ, or haustorium, of the parasitic
angiosperm Cuscuta, but are not equally well charted in young haustoria. In this study, we focused on the molecular
processes in the early stages of developing haustoria; that is, before the parasite engages in a physiological contact
with its host. We describe first the identification of differentially expressed genes in young haustoria whose development was induced by far-red light and tactile stimuli in the absence of a host plant by suppression subtractive
hybridization. To improve sequence information and to aid in the identification of the obtained candidates, reference
transcriptomes derived from two species of Cuscuta, C. gronovii and C. reflexa, were generated. Subsequent quantitative gene expression analysis with different tissues of C. reflexa revealed that among the genes that were up-regulated in young haustoria, two xyloglucan endotransglucosylase/hydrolase (XTH) genes were highly expressed almost
exclusively at the onset of haustorium development. The same expression pattern was also found for the closest XTH
homologues from C. gronovii. In situ assays for XTH-specific action suggested that xyloglucan endotransglucosylation was most pronounced in the cell walls of the swelling area of the haustorium facing the host plant, but was also
detectable in later stages of haustoriogenesis. We propose that xyloglucan remodelling by Cuscuta XTHs prepares
the parasite for host infection and possibly aids the invasive growth of the haustorium.
Forlag
Oxford University PressSitering
Olsen S, Ketelsen Striberny B, Hollmann, Schwacke R, Popper ZA, Krause K. Getting ready for host invasion: Elevated expression and action of xyloglucan endotransglucosylases/hydrolases in developing haustoria of the holoparasitic angiosperm Cuscuta. Journal of Experimental Botany. 2016;67(3):695-708Metadata
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