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dc.contributor.authorLiu, Yang
dc.contributor.authorAhator, Stephen Dela
dc.contributor.authorWang, Huishan
dc.contributor.authorFeng, Qishun
dc.contributor.authorXu, Yinuo
dc.contributor.authorLi, Chuhao
dc.contributor.authorZhou, Xiaofan
dc.contributor.authorZhang, Lian-Hui
dc.date.accessioned2022-09-07T07:17:08Z
dc.date.available2022-09-07T07:17:08Z
dc.date.issued2022-04-12
dc.description.abstractThe Pseudomonas aeruginosa strain PAO1 has routinely been used as a laboratory model for quorum sensing (QS). However, the microevolution of P. aeruginosa laboratory strains resulting in genetic and phenotypic variations have caused inconsistencies in QS research. To investigate the underlying causes of these variations, we analyzed 5 Pseudomonas aeruginosa PAO1 sublines from our laboratory using a combination of phenotypic characterization, high throughput genome sequencing, and bioinformatic analysis. The major phenotypic variations among the sublines spanned across the levels of QS signals and virulence factors such as pyocyanin and elastase. Furthermore, the sublines exhibited distinct variations in motility and biofilm formation. Most of the phenotypic variations were mapped to mutations in the lasR and mexT, which are key components of the QS circuit. By introducing these mutations in the subline PAO1-E, which is devoid of such mutations, we confirmed their influence on QS, virulence, motility, and biofilm formation. The findings further highlight a possible divergent regulatory mechanism between the LasR and MexT in the P. aeruginosa. The results of our study reveal the effects of microevolution on the reproducibility of most research data from QS studies and further highlight mexT as a key component of the QS circuit of P. aeruginosa.en_US
dc.identifier.citationLiu, Ahator, Wang, Feng, Xu, Li, Zhou, Zhang. Microevolution of the mexT and lasR Reinforces the Bias of Quorum Sensing System in Laboratory Strains of Pseudomonas aeruginosa PAO1. Frontiers in Microbiology. 2022;13en_US
dc.identifier.cristinIDFRIDAID 2029048
dc.identifier.doi10.3389/fmicb.2022.821895
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/10037/26691
dc.language.isoengen_US
dc.publisherFrontiers Mediaen_US
dc.relation.journalFrontiers in Microbiology
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.titleMicroevolution of the mexT and lasR Reinforces the Bias of Quorum Sensing System in Laboratory Strains of Pseudomonas aeruginosa PAO1en_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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