Vis enkel innførsel

dc.contributor.advisorFenton, Christopher Graham
dc.contributor.authorRay, Mithlesh Kumar
dc.date.accessioned2024-05-30T07:19:33Z
dc.date.available2024-05-30T07:19:33Z
dc.date.issued2024-06-12
dc.description.abstractUlcerative colitis (UC) is a chronic inflammatory condition of the large intestine, influenced by various factors such as genetics, environment, gut contents, and the host's immune system. It has been reported that epigenetic factors, such as long non-coding RNAs (lncRNAs) and DNA methylation, play a role in UC development. The contribution of lncRNAs to UC pathogenesis is not fully understood. Therefore, it is important to thoroughly study lncRNAs for their relevance in UC pathogenesis. This thesis aims to understand lncRNAs in UC and their potential impact on how the disease develops. In the initial study, a group of uncharacterized lncRNAs was identified, demonstrating the potential to distinguish UC patients from the control group. Subsequently, the second study found several UC-associated lncRNAs that might be regulated by DNA methylation. Methylation-regulated lncRNAs may play a regulatory role in the expression of genes associated with inflammation, immune responses, and intestinal barrier integrity. In the third study, data from publicly available datasets were combined to identify commonly differentially expressed lncRNAs in UC. Many lncRNAs associated with colorectal cancer (CRC) were found to be commonly differentially expressed in publicly available UC datasets. The study also highlighted the complexity associated with combining data from different studies. Altogether, this study highlights the potential contribution of lncRNAs in UC pathogenesis, adding to our understanding of the underlying molecular mechanisms of disease development. The results of this study may serve as the foundation for identifying diagnostic markers and therapeutic interventions.en_US
dc.description.doctoraltypeph.d.en_US
dc.description.popularabstractLong non-coding RNA (lncRNA) is a crucial player in cellular biology, holding a key role in development, gene expression regulation, and disease pathogenesis. This study employs high-throughput data and bioinformatics tools to investigate lncRNAs in ulcerative colitis (UC). Initially, the study identified a set of previously uncharacterized lncRNAs. These lncRNAs can distinguish UC patients from healthy individuals and may serve as potential diagnostic markers. Combining DNA methylation with lncRNA expression deepened our understanding of the molecular mechanisms underlying UC pathogenesis. The combination of data from independent studies in a publicly available dataset revealed several differentially expressed lncRNAs that are linked to colorectal cancer (CRC) development—a life-threatening risk for UC patients. These studies underscore the potential role of lncRNAs in UC, offering insights into disease mechanisms, diagnostic markers, and therapeutic avenues.en_US
dc.identifier.urihttps://hdl.handle.net/10037/33648
dc.language.isoengen_US
dc.publisherUiT The Arctic University of Norwayen_US
dc.publisherUiT Norges arktiske universiteten_US
dc.relation.haspart<p>Paper I: Ray, M.K., Fenton, C.G. & Paulssen, R.H. (2022). Novel long non-coding RNAs of relevance for ulcerative colitis pathogenesis. <i>Non-coding RNA research, 7</i>(1), 40–47. Also available in Munin at <a href=https://hdl.handle.net/10037/27285>https://hdl.handle.net/10037/27285</a>. <p>Paper II: Fenton, C.G., Ray, M.K., Meng, W. & Paulssen, R.H. (2023). Methylation-Regulated Long Non-Coding RNA Expression in Ulcerative Colitis. <i>International Journal of Molecular Sciences, 24</i>(13), 10500. Also available in Munin at <a href=https://hdl.handle.net/10037/29934>https://hdl.handle.net/10037/29934</a>. <p>Paper III: Ray, M.K., Fenton, C.G. & Paulssen, R.H. (2024). Challenges in defining a reference set of differentially expressed lncRNAs in ulcerative colitis by meta-analysis. <i>Current Issues in Molecular Biology, 46</i>(4), 3164-3174. Also available in Munin at <a href=https://hdl.handle.net/10037/33646>https://hdl.handle.net/10037/33646</a>.en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2024 The Author(s)
dc.subject.courseIDDOKTOR-003
dc.titleLong non-coding RNAs in ulcerative colitisen_US
dc.typeDoctoral thesisen_US
dc.typeDoktorgradsavhandlingen_US


Tilhørende fil(er)

Thumbnail
Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel