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dc.contributor.authorRanjan, Abhishek
dc.contributor.authorSwain, Jaya Kumari
dc.contributor.authorAhluwalia, Balpreet Singh
dc.contributor.authorMelandsø, Frank
dc.date.accessioned2024-11-12T08:12:22Z
dc.date.available2024-11-12T08:12:22Z
dc.date.issued2024-10-22
dc.description.abstractSignificance Three-dimensional photoacoustic imaging (PAM) has emerged as a promising technique for non-invasive label-free visualization and characterization of biological tissues with high spatial resolution and functional contrast.<p> <p>Aim The application of PAM and ultrasound as a microscopy technique of study for Atlantic salmon skin is presented here. <p>Approach A custom ultrasound and photoacoustic experimental setup was used for conducting this experiment with a sample preparation method where the salmon skin is embedded in aga rose and lifted from the bottom of the petridish. <p>Results The results of C-scan, B-scan, and overlayed images of ultrasound and photoacoustic are presented. The results are then analyzed for understanding the pigment map and its relation to salmon behavior to external stimuli. The photoacoustic images are compared with the optical images and analyzed further. A custom colormap and alpha map is designed and the matrices responsible for PAM and ultrasound are inserted together to overlay the ultrasound image and PAM image on top of each other. <p>Conclusions In this study, we propose an approach that combines scanning acoustic microscopy (SAM) images with PAM images for providing a comprehensive understanding of the salmon skin tissue. Overlaying acoustic and photoacoustic images enabled unique visualization of tissue morphology, with respect to identification of structural features in the context of their pigment distribution.en_US
dc.identifier.citationRanjan, Swain, Ahluwalia, Melandsø. 3-D Visualization of Atlantic salmon skin through Ultrasound and Photoacoustic Microscopy. PLoS Computational Biology. 2024;20(10):e1011709en_US
dc.identifier.cristinIDFRIDAID 2316268
dc.identifier.doi10.1371/journal.pcbi.1011709
dc.identifier.issn1553-734X
dc.identifier.issn1553-7358
dc.identifier.urihttps://hdl.handle.net/10037/35657
dc.language.isoengen_US
dc.publisherPLOSen_US
dc.relation.journalPLoS Computational Biology
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2024 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.title3-D Visualization of Atlantic salmon skin through Ultrasound and Photoacoustic Microscopyen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Med mindre det står noe annet, er denne innførselens lisens beskrevet som Attribution 4.0 International (CC BY 4.0)