<i>Listeria monocytogenes</i> is a common food-borne pathogen that can disseminate from the intestine and infect multiple organs. Here, we used sequence tag-based analysis of microbial populations (STAMP) to investigate <i>L. monocytogenes</i> population dynamics during infection. We created a genetically barcoded library of murinized <i>L. monocytogenes</i> and then used deep sequencing to track ...