Vis enkel innførsel

dc.contributor.authorPope, Phillip B
dc.contributor.authorMackenzie, Alasdair
dc.contributor.authorGregor, Ivan
dc.contributor.authorSmith, Wendy
dc.contributor.authorSundset, Monica Alterskjær
dc.contributor.authorMcHardy, Alice
dc.contributor.authorMorrison, Mark
dc.contributor.authorEijsink, Vincent
dc.date.accessioned2012-12-17T10:20:28Z
dc.date.available2012-12-17T10:20:28Z
dc.date.issued2012
dc.description.abstractLignocellulosic biomass remains a largely untapped source of renewable energy predominantly due to its recalcitrance and an incomplete understanding of how this is overcome in nature. We present here a compositional and comparative analysis of metagenomic data pertaining to a natural biomass-converting ecosystem adapted to austere arctic nutritional conditions, namely the rumen microbiome of Svalbard reindeer (Rangifer tarandus platyrhynchus). Community analysis showed that deeply-branched cellulolytic lineages affiliated to the Bacteroidetes and Firmicutes are dominant, whilst sequence binning methods facilitated the assemblage of metagenomic sequence for a dominant and novel Bacteroidales clade (SRM-1). Analysis of unassembled metagenomic sequence as well as metabolic reconstruction of SRM-1 revealed the presence of multiple polysaccharide utilization loci-like systems (PULs) as well as members of more than 20 glycoside hydrolase and other carbohydrate-active enzyme families targeting various polysaccharides including cellulose, xylan and pectin. Functional screening of cloned metagenome fragments revealed high cellulolytic activity and an abundance of PULs that are rich in endoglucanases (GH5) but devoid of other common enzymes thought to be involved in cellulose degradation. Combining these results with known and partly re-evaluated metagenomic data strongly indicates that much like the human distal gut, the digestive system of herbivores harbours high numbers of deeply branched and as-yet uncultured members of the Bacteroidetes that depend on PUL-like systems for plant biomass degradation.en
dc.identifier.citationPLoS ONE 7 (2012), vol. 6: e38571.en
dc.identifier.cristinIDFRIDAID 928471
dc.identifier.doidoi: 10.1371/journal.pone.0038571
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/10037/4683
dc.identifier.urnURN:NBN:no-uit_munin_4394
dc.language.isoengen
dc.publisherPublic Library of Science (PLoS)en
dc.rights.accessRightsopenAccess
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480::Zoophysiology and comparative physiology: 483en
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Zoofysiologi og komparativ fysiologi: 483en
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480::Zoogeography: 486en
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Zoogeografi: 486en
dc.titleMetagenomics of the Svalbard Reindeer Rumen Microbiome Reveals Abundance of Polysaccharide Utilization Locien
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


Tilhørende fil(er)

Thumbnail
Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel