• Chip-based multimodal super-resolution microscopy for histological investigations of cryopreserved tissue sections 

      Villegas, Luis; Dubey, Vishesh Kumar; Nystad, Mona; Tinguely, Jean-Claude; Coucheron, David Andre; Dullo, Firehun Tsige; Priyadarshi, Anish; Acuna Maldonado, Sebastian Andres; Ahmad, Azeem; Mateos, Jose M.; Barmettler, Gery; Ziegler, Urs; Birgisdottir, Åsa Birna; Hovd, Aud-Malin Karlsson; Fenton, Kristin Andreassen; Acharya, Ganesh; Agarwal, Krishna; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-02-24)
      Histology involves the observation of structural features in tissues using a microscope. While diffraction-limited optical microscopes are commonly used in histological investigations, their resolving capabilities are insufficient to visualize details at subcellular level. Although a novel set of super-resolution optical microscopy techniques can fulfill the resolution demands in such cases, the ...
    • High-throughput spatial sensitive quantitative phase microscopy using low spatial and high temporal coherent illumination 

      Ahmad, Azeem; Dubey, Vishesh; Jayakumar, Nikhil; Habib, Anowarul; Butola, Ankit; Nystad, Mona; Acharya, Ganesh; Basnet, Purusotam; Mehta, Dalip Singh; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-08-04)
      High space-bandwidth product with high spatial phase sensitivity is indispensable for a single-shot quantitative phase microscopy (QPM) system. It opens avenue for widespread applications of QPM in the field of biomedical imaging. Temporally low coherence light sources are implemented to achieve high spatial phase sensitivity in QPM at the cost of either reduced temporal resolution or smaller field ...
    • Photonic-chip: a multimodal imaging tool for histopathology 

      Villegas, Luis; Dubey, Vishesh Kumar; Tinguely, Jean-Claude; Coucheron, David Andre; Priyadarshi, Anish; Acuña Maldonado, Sebastian Andres; Agarwal, Krishna; Mateos, Jose M; Nystad, Mona; Hovd, Aud-Malin Karlsson; Fenton, Kristin Andreassen; Ahluwalia, Balpreet Singh (Conference object; Konferansebidrag, 2021-04)
      We propose the photonic-chip as a multimodal imaging platform for histopathological assessment, allowing large fields-of-view across diverse microscopy methods including total internal reflection fluorescence and single-molecule localization.
    • Visualizing ultrastructural details of placental tissue with super-resolution structured illumination microscopy 

      Villegas Hernandez, Luis Enrique; Nystad, Mona; Ströhl, Florian; Basnet, Purusotam; Acharya, Ganesh; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-14)
      Super-resolution fluorescence microscopy is a widely employed technique in cell biology research, yet remains relatively unexplored in the field of histopathology. Here, we describe the sample preparation steps and acquisition parameters necessary to obtain fluorescent multicolor super-resolution structured illumination microscopy (SIM) images of both formalin-fixed paraffin-embedded and cryo-preserved ...