Image denoising or artefact removal using deep learning is possible in the availability of supervised training dataset acquired in real experiments or synthesized using known noise models. Neither of the conditions can be fulfilled for nanoscopy (super-resolution optical microscopy) images that are generated from microscopy videos through statistical analysis techniques. Due to several physical ...

Photonic-chip-based light illumination has recently found applications in optical microscopy and nanoscopy methodologies. The photonic chip removes the dependency on imaging objective lenses to generate the required illumination patterns for different microscopy methods. Until now, all the reported chip-based optical microscopy methods exploit the evanescent field present on top of a waveguide surface ...

Multiple signal classification algorithm (MUSICAL) exploits temporal fluctuations in fluorescence intensity to perform super-resolution microscopy by computing the value of a super-resolving indicator function across a fine sample grid. A key step in the algorithm is the separation of the measurements into signal and noise subspaces, based on a single user-specified parameter called the threshold. ...

New quantitative and semiautomated methods for analyzing oil slick evolution using a time series of L-band synthetic aperture radar (SAR) images with short repeat time are developed and explored. In this study, two methods that are complementary in terms of identifying temporal changes within an oil slick are presented. The two methods reflect two ways of evaluating the oil slicks. The first method ...

In recent years, space-borne synthetic aperture radar (SAR) polarimetry has become a valuable tool for sea ice type retrieval. L-band SAR has proven to be sensitive toward deformed sea ice and is complementary compared with operationally used C-band SAR for sea ice type classification during the early and advanced melt seasons. Here, we employ an artificial neural network (ANN)-based sea ice type ...

A highly sensitive integrated photonic transducer is designed by utilizing asymmetric long-period gratings on a silicon waveguide. These gratings are formed by periodic perturbation of the waveguide width, leading to coupling between the fundamental mode and the 1st order asymmetric leaky mode. The coupled modes are studied via finite-element and finite-difference time-domain methods. Only a single ...

A layer-by-layer deposition method for fabricating a focused ultrasonic transducer from piezoelectric copolymers has been developed. The fabrication process involves engraving a spherical cavity of 2 mm diameter on polyethyleneimines (PEI) polymer substrate. Surface roughness of the engraved spherical cavity is measured and compared with simulated line scan. Then, the transducer response was ...

Immunofluorescence microscopy is routinely used in the diagnosis of and research on renal impairments. However, this highly specific technique is restricted in its maximum resolution to about 250 nm in the lateral and 700 nm in the axial directions and thus not sufficient to investigate the fine subcellular structure of the kidney’s glomerular filtration barrier. In contrast, electron microscopy ...

Quantitative phase microscopy (QPM) is a label-free technique that enables monitoring of morphological changes at the subcellular level. The performance of the QPM system in terms of spatial sensitivity and resolution depends on the coherence properties of the light source and the numerical aperture (NA) of objective lenses. Here, we propose high space-bandwidth quantitative phase imaging using ...

Photonic-chip based TIRF illumination has been used to demonstrate several on-chip optical nanoscopy methods. The sample is illuminated by the evanescent field generated by the electromagnetic wave modes guided inside the optical waveguide. In addition to the photokinetics of the fluorophores, the waveguide modes can be further exploited for introducing controlled intensity fluctuations for exploitation ...