The point spread function of a fixed fluorophore with its dipole axis colinear to the optical axis appears donut-shaped when seen through a microscope, and its light distribution in the pupil plane is radially polarized. Yet other techniques, such as photolithography, report that this same light distribution in the pupil plane appears as a solid spot. How can this same distribution lead to a spot ...

Here, we discuss the effects that the dynamics of the hydration layer and other variables, such as the tip radius, have on the availability of imaging regimes in dynamic AFM—including multifrequency AFM. Since small amplitudes are required for high-resolution imaging, we focus on these cases. It is possible to fully immerse a sharp tip under the hydration layer and image with amplitudes similar to ...

An unusual, large, latent-heat polynya opened and then closed by freezing and convergence north of Greenland’s coast in late winter 2018. The closing presented a natural but well-constrained full-scale ice deformation experiment. We observed the closing of and deformation within the polynya with satellite synthetic-aperture radar (SAR) imagery and measured the accumulated effects of dynamic ...

There is a growing interest in automatic crafting of neural network architectures as opposed to expert tuning to fnd the best architecture. On the other hand, the problem of stock trading is considered one of the most dynamic systems that heavily depends on complex trends of the individual company. This paper proposes a novel self-evolving neural network system called self-evolving Multi-Layer ...

The Ionospheric Continuous-wave E-region Bistatic Experimental Auroral Radar (ICEBEAR) is a VHF coherent scatter radar that operates with a field-of-view centered on 58°N, 106°W and measures characteristics of ionospheric E-region plasma density irregularities. The initial operations of ICEBEAR utilized a wavelength-spaced linear receiving array to determine the angle of arrival of the ionospheric ...

A growing number of studies are concluding that the resilience of the Arctic sea ice cover in a warming climate is essentially controlled by its thickness. Satellite radar and laser altimeters have allowed us to routinely monitor sea ice thickness across most of the Arctic Ocean for several decades. However, a key uncertainty remaining in the sea ice thickness retrieval is the error on the sea surface ...

Visualization of three-dimensional (3D) morphological changes in the subcellular structures of a biological specimen is a major challenge in life science. Here, we present an integrated chip-based optical nanoscopy combined with quantitative phase microscopy (QPM) to obtain 3D morphology of liver sinusoidal endothelial cells (LSEC). LSEC have unique morphology with small nanopores (50-300 nm in ...

Segmenting subcellular structures in living cells from fluorescence microscope images is a ground truth (GT)-deficient problem. The microscopes’ three-dimensional blurring function, finite optical resolution due to light diffraction, finite pixel resolution and the complex morphological manifestations of the structures all contribute to GT-hardness. Unsupervised segmentation approaches are quite ...

The performance of piezoelectric sensors deteriorated due to the presence of defect, delamination, and corrosion that needed to be diagnosed for the effective implementation of the structural health monitoring (SHM) framework. A novel experimental approach based on Coulomb coupling is devised to visualise the interaction of ultrasonic waves with microscale defects in the Lead Zirconate Titanate ...

This paper presents and discusses electron density depletion regions observed with the incoherent scatter EISCAT Svalbard Radar (ESR) located at 75.43°N geomagnetic latitude. The data include several decades of measurements, which make them suitable for studying statistical features and characteristics of the ionospheric parameters. Here we focus on the electron density depletions and their dependence ...