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dc.contributor.authorIsemer, Rena
dc.contributor.authorKrause, Kirsten
dc.contributor.authorGrabe, Nils
dc.contributor.authorKitahata, Nobutaka
dc.contributor.authorAsami, Tadao
dc.contributor.authorKrupinska, Karin
dc.date.accessioned2013-03-13T14:26:49Z
dc.date.available2013-03-13T14:26:49Z
dc.date.issued2012
dc.description.abstractWHIRLY1 is a protein that can be translocated from the plastids to the nucleus, making it an ideal candidate for communicating information between these two compartments. Mutants of Arabidopsis thaliana lacking WHIRLY1 (why1) were shown to have a reduced sensitivity toward salicylic acid (SA) and abscisic acid (ABA) during germination. Germination assays in the presence of abamine, an inhibitor of ABA biosynthesis, revealed that the effect of SA on germination was in fact caused by a concomitant stimulation of ABA biosynthesis. In order to distinguish whether the plastid or the nuclear isoform of WHIRLY1 is adjusting the responsiveness toward ABA, sequences encoding either the complete WHIRLY1 protein or a truncated form lacking the plastid transit peptide were overexpressed in the why1 mutant background. In plants overexpressing the full-length sequence, WHIRLY1 accumulated in both plastids and the nucleus, whereas in plants overexpressing the truncated sequence, WHIRLY1 accumulated exclusively in the nucleus. Seedlings containing recombinant WHIRLY1 in both compartments were hypersensitive toward ABA. In contrast, seedlings possessing only the nuclear form of WHIRLY1 were as insensitive toward ABA as the why1 mutants. ABA was furthermore shown to lower the rate of germination of wildtype seeds even in the presence of abamine which is known to inhibit the formation of xanthoxin, the plastid located precursor of ABA. From this we conclude that plastid located WHIRLY1 enhances the responsiveness of seeds toward ABA even when ABA is supplied exogenously.en
dc.identifier.citationFrontiers in Plant Science 3(2012) nr. 283en
dc.identifier.cristinIDFRIDAID 970835
dc.identifier.doihttp://dx.doi.org/10.3389/fpls.2012.00283
dc.identifier.issn1664-462X
dc.identifier.urihttps://hdl.handle.net/10037/5002
dc.identifier.urnURN:NBN:no-uit_munin_4695
dc.language.isoengen
dc.rights.accessRightsopenAccess
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480::Plant physiology: 492en
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Plantefysiologi: 492en
dc.titlePlastid located WHIRLY1 enhances the responsiveness of Arabidopsis seedlings toward abscisic aciden
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


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