Blar i forfatter Norges arktiske universitetsmuseum "Kirchefer, Andreas"

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    • Shotgun-sequencing of plant DNA: Selection og material and methods 

      Merkel, Marie Kristine Føreid; Pitelkova, Iva; Lammers, Youri; Coissac, Eric; Alberti, Adriana; Dossat, Carole; Kirchefer, Andreas; Alsos, Inger Greve (Conference object; Konferansebidrag, 2017)
      Initially, rbcLaand ITS2 were PCR amplified and Sanger sequenced for 564 species from 1900 specimens. To increase the amount of the genome covered, shotgun sequencing of 1670 species is in progress. Our Sanger analyses had a success rate of 74% and 85% for ITS2 and rbcLa, respectively. Overall success rate of Sanger sequencing decreased with later collection date in the year. Preliminary results of ...