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dc.contributor.authorAarnes, Siv Grethe
dc.contributor.authorFløystad, Ida
dc.contributor.authorSchregel, Julia
dc.contributor.authorVindstad, Ole Petter L.
dc.contributor.authorJepsen, Jane Uhd
dc.contributor.authorEiken, Hans Geir
dc.contributor.authorIms, Rolf Anker
dc.contributor.authorHagen, Snorre
dc.date.accessioned2015-09-28T11:08:28Z
dc.date.available2015-09-28T11:08:28Z
dc.date.issued2015-09-17
dc.description.abstractThe autumnal moth (Epirrita autumnata) is a cyclically outbreaking forest Lepidoptera with circumpolar distribution and substantial impact on Northern ecosystems. We have isolated 21 microsatellites from the species to facilitate population genetic studies of population cycles, outbreaks, and crashes. First, PCR primers and PCR conditions were developed to amplify 19 trinucleotide loci and two tetranucleotide loci in six multiplex PCR approaches and then analyzed for species specificity, sensitivity and precision. Twelve of the loci showed simple tandem repeat array structures while nine loci showed imperfect repeat structures, and repeat numbers varied in our material between six and 15. The application in population genetics for all the 21 microsatellites were further validated in 48 autumnal moths sampled from Northern Norway, and allelic variation was detected in 19 loci. The detected numbers of alleles per locus ranged from two to 13, and the observed and expected heterozygosities varied from 0.04 to 0.69 and 0.04 to 0.79, respectively. Evidence for linkage disequilibrium was found for six loci as well as indication of one null allele. We find that these novel microsatellites and their multiplex-PCR assays are suitable for further research on fine- and large-scale population-genetic studies of Epirrita autumnata. tri- and tetranucleotide microsatellites; multiplex PCR; Lepidoptera; population geneticsen_US
dc.identifier.citationInternational Journal of Molecular Sciences 16(2015) s. 22541-22554en_US
dc.identifier.cristinIDFRIDAID 1266899
dc.identifier.doi10.3390/ijms160922541
dc.identifier.issn1422-0067
dc.identifier.urihttps://hdl.handle.net/10037/8142
dc.identifier.urnURN:NBN:no-uit_munin_7730
dc.language.isoengen_US
dc.rights.accessRightsopenAccess
dc.subjecttri- and tetranucleotide microsatellitesen_US
dc.subjectmultiplex PCRen_US
dc.subjectLepidopteraen_US
dc.subjectpopulation geneticsen_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473en_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Molecular biology: 473en_US
dc.titleIdentification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae)en_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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