ub.xmlui.mirage2.page-structure.muninLogoub.xmlui.mirage2.page-structure.openResearchArchiveLogo
    • EnglishEnglish
    • norsknorsk
  • Velg spraakEnglish 
    • EnglishEnglish
    • norsknorsk
  • Administration/UB
View Item 
  •   Home
  • Fakultet for biovitenskap, fiskeri og økonomi
  • Institutt for arktisk og marin biologi
  • Artikler, rapporter og annet (arktisk og marin biologi)
  • View Item
  •   Home
  • Fakultet for biovitenskap, fiskeri og økonomi
  • Institutt for arktisk og marin biologi
  • Artikler, rapporter og annet (arktisk og marin biologi)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Brucella pinnipedialis in hooded seal (Cystophora cristata) primary epithelial cells

Permanent link
https://hdl.handle.net/10037/8578
DOI
https://doi.org/10.1186/s13028-016-0188-5
Thumbnail
View/Open
article.pdf (1.350Mb)
(PDF)
Date
2016-01-25
Type
Journal article
Tidsskriftartikkel
Peer reviewed

Author
Larsen, Anett Kristin; Godfroid, Jacques; Nymo, Ingebjørg Helena
Abstract
Background: Marine Brucella spp. have been isolated from numerous pinniped and cetacean species, but pathological findings in association with infection with Brucella pinnipedialis in pinnipeds have been sparse. The capacity of brucellae to survive and replicate within host macrophages underlies their important ability to produce chronic infections, but previous work has shown that B. pinnipedialis spp. are rapidly eliminated from hooded seal (Cystophora cristata) alveolar macrophages.

Results: To investigate if multiplication could take place in other hooded seal cell types, primary epithelial cells were isolated, verified to express the epithelial marker cytokeratin and challenged with three different strains of B. pinnipedialis; B. pinnipedialis sp. nov., B. pinnipedialis hooded seal strain B17, and B. pinnipedialis hooded seal strain 22F1. All strains were steadily eliminated and the amounts of intracellular bacteria were reduced to less than one-third by 48 h post infection. Intracellular presence was verified using immunocytochemistry.

Conclusions: So far, intracellular multiplication in seal cells has not been documented for B. pinnipedialis. The lack of intracellular survival in macrophages, as well as in epithelial cells, together with the fact that pathological changes due to B. pinnipedialis infection is not yet identified in seals, suggests that the bacteria may only cause a mild, acute and transient infection. These findings also contribute to substantiate the hypothesis that seals may not be the primary host of B. pinnipedialis and that the transmission to seals are caused by other species in the marine environment.

Description
License:Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)
Publisher
BioMed Central
Citation
Acta Veterinaria Scandinavica 2016, 58(9)
Metadata
Show full item record
Collections
  • Artikler, rapporter og annet (arktisk og marin biologi) [1630]

Browse

Browse all of MuninCommunities & CollectionsAuthor listTitlesBy Issue DateBrowse this CollectionAuthor listTitlesBy Issue Date
Login

Statistics

View Usage Statistics
UiT

Munin is powered by DSpace

UiT The Arctic University of Norway
The University Library
uit.no/ub - munin@ub.uit.no

Accessibility statement (Norwegian only)