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dc.contributor.authorLarsen, Anett Kristin
dc.contributor.authorGodfroid, Jacques
dc.contributor.authorNymo, Ingebjørg Helena
dc.date.accessioned2016-02-26T14:45:23Z
dc.date.available2016-02-26T14:45:23Z
dc.date.issued2016-01-25
dc.description.abstractBackground: Marine Brucella spp. have been isolated from numerous pinniped and cetacean species, but pathological findings in association with infection with Brucella pinnipedialis in pinnipeds have been sparse. The capacity of brucellae to survive and replicate within host macrophages underlies their important ability to produce chronic infections, but previous work has shown that B. pinnipedialis spp. are rapidly eliminated from hooded seal (Cystophora cristata) alveolar macrophages. <p>Results: To investigate if multiplication could take place in other hooded seal cell types, primary epithelial cells were isolated, verified to express the epithelial marker cytokeratin and challenged with three different strains of B. pinnipedialis; B. pinnipedialis sp. nov., B. pinnipedialis hooded seal strain B17, and B. pinnipedialis hooded seal strain 22F1. All strains were steadily eliminated and the amounts of intracellular bacteria were reduced to less than one-third by 48 h post infection. Intracellular presence was verified using immunocytochemistry. <p>Conclusions: So far, intracellular multiplication in seal cells has not been documented for B. pinnipedialis. The lack of intracellular survival in macrophages, as well as in epithelial cells, together with the fact that pathological changes due to B. pinnipedialis infection is not yet identified in seals, suggests that the bacteria may only cause a mild, acute and transient infection. These findings also contribute to substantiate the hypothesis that seals may not be the primary host of B. pinnipedialis and that the transmission to seals are caused by other species in the marine environment.en_US
dc.descriptionLicense:Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)en_US
dc.identifier.citationActa Veterinaria Scandinavica 2016, 58(9)en_US
dc.identifier.cristinIDFRIDAID 1325052
dc.identifier.doi10.1186/s13028-016-0188-5
dc.identifier.issn1751-0147
dc.identifier.urihttps://hdl.handle.net/10037/8578
dc.identifier.urnURN:NBN:no-uit_munin_8143
dc.language.isoengen_US
dc.publisherBioMed Centralen_US
dc.rights.accessRightsopenAccess
dc.subjectBrucella pinnipedialisen_US
dc.subjectEpithelial cellsen_US
dc.subjectHooded sealen_US
dc.subjectInfectionen_US
dc.subjectIn vitroen_US
dc.subjectMarine ecosystemen_US
dc.subjectPinnipedsen_US
dc.subjectSpilloveren_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497en_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497en_US
dc.titleBrucella pinnipedialis in hooded seal (Cystophora cristata) primary epithelial cellsen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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