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dc.contributor.authorSeternes, Tore
dc.contributor.authorTonheim, Tom Christian
dc.contributor.authorMyhr, Anne Ingeborg
dc.contributor.authorDalmo, Roy Ambli
dc.date.accessioned2017-03-16T13:55:18Z
dc.date.available2017-03-16T13:55:18Z
dc.date.issued2016
dc.description.abstractThe long-term persistence and activity of a naked plasmid DNA (pGL3-35S) containing a luc gene (reporter gene) controlled by a plant 35S CaMV promoter was studied in Atlantic salmon (Salmo salar L.) after injection. Atlantic salmon (mean weight 70 grams) were injected intramuscularly with 100 μg of plasmid DNA. Blood, different tissues and organs were sampled at different time points up to day 535 after injection. Southern blot analysis suggested the presence of extra-chromosomally open circular, linear and supercoiled topoforms of pGL3-35S at day 150 after injection. At day 536 open circular and supercoiled topoforms were detected. Luciferase activity was detected at the injection site up to 536 days post-injection of pGL3-35S, where it peaked at day 150 and decreased to approximately 17% of its maximum activity by day 536. Our study demonstrated that a plasmid containing the 35S promoter was able to induce expression of a reporter gene/protein in fish in vivo and that the plasmid DNA persisted for a prolonged time after intramuscular injection.en_US
dc.description.sponsorshipThe present study was supported by The National Program for Research in Functional Genomics in Norway (FUGE) in the Research Council of Norway [grant no 157157/150 to AIM], Environment and Development: Biological diversity - Dynamics, Threats and Management, [grant no. 165144/S30 to AIM]. Furthermore the Research Council of Norway [through grant number 182035 and 183204/S40 to RAD], and Tromsø Research Foundation (“Induction and assessment of T cell immunity to virus antigens in salmonids” to RAD) have also contributed to funding. Funding for open access charge: [University of Tromsø].en_US
dc.descriptionPublished version. Source at <a href=http://doi.org/10.1038/srep25096>http://doi.org/10.1038/srep25096</a>. License <a href=https://creativecommons.org/licenses/by/4.0/>CC BY 4.0</a>.en_US
dc.identifier.citationSeternes T, Tonheim TC, Myhr A I, Dalmo RA. A plant 35S CaMV promoter induces long-term expression of luciferase in Atlantic salmon. Scientific Reports. 2016;6en_US
dc.identifier.cristinIDFRIDAID 1389885
dc.identifier.doi10.1038/srep25096
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/10037/10741
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.relation.journalScientific Reports
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FundingProgram/157157/Norway//FUGE/en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FundingProgram/165144/Norway///en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FundingProgram/182035/Norway///en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FundingProgram/183204/Norway///en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920en_US
dc.subjectgenetic vectors
dc.titleA plant 35S CaMV promoter induces long-term expression of luciferase in Atlantic salmonen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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