• Estuarine molecular bycatch as a landscape-wide biomonitoring tool 

      Mariani, Stefano; Harper, Linsey R.; Collins, Rupert A.; Baillie, Charles; Wangensteen, Owen S.; McDevitt, Allan D.; Heddell-Cowie, Morton; Genner, Martin J. (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-08-13)
      Environmental DNA analysis is rapidly transforming biodiversity monitoring and bolstering conservation applications worldwide. This approach has been assisted by the development of metabarcoding PCR primers that are suited for detection of a wide range of taxa. However, little effort has gone into exploring the value of the non-target DNA sequences that are generated in every survey, but subsequently ...
    • Non‐specific amplification compromises environmental DNA metabarcoding with COI 

      Collins, Rupert A.; Bakker, Judith; Wangensteen Fuentes, Owen Simon; Soto, Ana Z.; Corrigan, Laura; Sims, David W.; Genner, Martin J.; Mariani, Stefano (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-08-02)
      <ol> <li>Metabarcoding extra‐organismal DNA from environmental samples is now a key technique in aquatic biomonitoring and ecosystem health assessment. Of critical consideration when designing experiments, and especially so when developing community standards and legislative frameworks, is the choice of genetic marker and primer set. Mitochondrial cytochrome c oxidase subunit I (COI), the standard ...
    • Persistence of environmental DNA in marine systems 

      Collins, Rupert A.; Wangensteen Fuentes, Owen Simon; O'Gorman, Eoin J.; Mariani, Stefano; Sims, David W.; Genner, Martin J. (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-11-05)
      As environmental DNA (eDNA) becomes an increasingly valuable resource for marine ecosystem monitoring, understanding variation in its persistence across contrasting environments is critical. Here, we quantify the breakdown of macrobial eDNA over a spatio-temporal axis of locally extreme conditions, varying from ocean-influenced offshore to urban-inshore, and between winter and summer. We report that ...