Methanol is an attractive alternative non-food feedstock for industrial fermentations that can be used instead of sugar-based raw materials. Here, the thermophilic and methylotrophic bacterium <i>Bacillus methanolicus</i> MGA3 was metabolically engineered to produce the platform chemical (<i>R</i>)-acetoin from methanol at 50 °C. Three different heterologous <i>alsSD/budAB</i> operons, each encoding ...