Blar i forfatter Artikler, rapporter og annet (Norges fiskerihøgskole) "Chang, Chia Jung"

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    • Adjuvant activity of fish type I interferon shown in a virus DNA vaccination model 

      Chang, Chia Jung; Sun, Baojian; Robertsen, Børre (Journal article; Tidsskriftartikkel; Peer reviewed, 2015-04-09)
      There is a need for more efficient vaccines to combat viral diseases of Atlantic salmon and other farmed fish. DNA vaccines are highly effective against salmonid rhabdoviruses, but have shown less effect against other viruses. In the present work we have studied if type I IFNs might be used as adjuvants in fish DNA vaccines. For this purpose we chose a DNA vaccine model based on the hemagglutin ...

    • Infectious pancreatic necrosis virus proteins VP2, VP3, VP4 and VP5 antagonize IFNa1 promoter activation while VP1 induces IFNa1 

      Lauksund, Reidunn Silje; Greiner-Tollersrud, Linn; Chang, Chia Jung; Robertsen, Børre (Journal article; Tidsskriftartikkel; Peer reviewed, 2015)
      Infectious pancreatic necrosis virus (IPNV) is one of the major viral pathogens causing disease in farmed Atlantic salmon worldwide. In the present work we show that several of the IPN proteins have powerful antagonistic properties against type I IFN induction in Atlantic salmon. Each of the five IPNV genes cloned into an expression vector were tested for the ability to influence activation of the ...

    • Protection of Atlantic salmon against virus infection by intramuscular injection of IFNc expression plasmid 

      Chang, Chia Jung; Robertsen, Camilla; Sun, Baojian; Robertsen, Børre (Journal article; Tidsskriftartikkel; Peer reviewed, 2014-07-02)
      n this work we have tested the in vivo antiviral activity of type I interferons (IFNs) in Atlantic salmon by injecting presmolts intramuscularly (i.m.) with plasmids encoding IFNa1, IFNb or IFNc under the control of a CMV promoter, and measured expression of antiviral genes in organs and protection against infection with infectious salmon anemia virus (ISAV)infection. Allthree IFN plasmids induced ...

    • Transcriptome analysis of plasmid-induced genes sheds light on the role of type I IFN as adjuvant in DNA vaccine against infectious salmon anemia virus 

      Sobhkhez, Mehrdad; Krasnov, Aleksei; Chang, Chia Jung; Robertsen, Børre (Journal article; Tidsskriftartikkel; Peer reviewed, 2017-11-21)
      A previous study showed that a plasmid expressing IFNa (pIFNa) strongly enhanced protection and antibody production of a DNA vaccine against infectious salmon anemia virus (ISAV) in Atlantic salmon. The vaccine consisted of a plasmid (pHE) expressing the virus hemagglutinin-esterase as an antigen. To increase the understanding of the adjuvant effect of pIFNa, we here compared transcriptome responses ...