• Adding zooplankton to the OSMAC toolkit: Effect of grazing stress on the metabolic profile and bioactivity of a diatom 

      Osvik, Renate Døving; Ingebrigtsen, Richard Andre; Norrbin, Fredrika; Andersen, Jeanette Hammer; Eilertsen, Hans Christian; Hansen, Espen Holst (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-02-03)
      “One strain many compounds” (OSMAC) based approaches have been widely used in the search for bioactive compounds. Introducing stress factors like nutrient limitation, UV-light or cocultivation with competing organisms has successfully been used in prokaryote cultivation. It is known that diatom physiology is affected by changed cultivation conditions such as temperature, nutrient concentration and ...
    • Bioactivity of a Marine Diatom (Porosira glacialis [Grunow] Jorgensen 1905) Cultivated With and Without Factory Smoke CO2 

      Osvik, Renate Døving; Andersen, Jeanette Hammer; Eilertsen, Hans Christian; Genevière, Anne-Marie; Hansen, Espen Holst (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-02-16)
      Using industrial emissions as a strategy for CO<sub>2</sub> sequestration through carbon capture and utilization (CCU) in cultivation of microalgae can potentially change cultivation factors such as pH, nutrient availability and presence of trace metals, which could alter the growth and metabolism of the microalgae. It is therefore important to investigate whether such changes in culturing conditions ...
    • Drivers of Atmosphere-Ocean CO2 Flux in Northern Norwegian Fjords 

      Aalto, Nerea Johanna; Campbell, Karley; Eilertsen, Hans Christian; Bernstein, Hans Christopher (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-07-07)
      High-latitude fjords and continental shelves are shown to be sinks for atmospheric CO2, yet large spatial-temporal variability and poor regional coverage of sea-air CO2 flux data, especially from fjord systems, makes it difficult to scale our knowledge on how they contribute to atmospheric carbon regulation. The magnitude and seasonal variability of atmosphere-sea CO2 flux was investigated in ...
    • Field sampling marine plankton for biodiscovery 

      Ingebrigtsen, Richard Andre; Hansen, Espen; Andersen, Jeanette Hammer; Eilertsen, Hans Christian (Journal article; Tidsskriftartikkel; Peer reviewed, 2017-11-20)
      Microalgae and plankton can be a rich source of bioactivity. However, induction of secondary metabolite production in lab conditions can be difficult. One simple way of bypassing this issue is to collect biomass in the field and screen for bioactivity. Therefore, bulk net samples from three areas along the coast of northern Norway and Spitsbergen were collected, extracted and fractionated. ...
    • Inclusion of photoautotrophic cultivated diatom biomass in salmon feed can deter lice 

      Eilertsen, Hans Christian; Elvevoll, Edel O.; Giæver, Ingeborg Hulda; Svenning, Jon Brage; Dalheim, Lars; Svalheim, Ragnhild Aven; Vang, Birthe; Siikavuopio, Sten Ivar; Dragøy, Ragnhild; Ingebrigtsen, Richard Andre; Hansen, Espen Holst; Hustad, Anette; Eilertsen, Karl-Erik (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-07-29)
      The aim of this study was to evaluate the potential of diatom (microalgae) biomass as a lice-reducing ingredient in salmon feed. The original hypothesis was based on the fact that polyunsaturated aldehydes (PUAs), e.g. 2-trans, 4-trans decadenial (A3) produced by diatoms can function as grazing deterrents and harm copepod development. Salmon lice (Lepeophtheirus salmonis) is a copepod, and we intended ...
    • Mass Cultivation of Microalgae: I. Experiences with Vertical Column Airlift Photobioreactors, Diatoms and CO2 Sequestration 

      Eilertsen, Hans Christian; Eriksen, Gunilla; Bergum, John-Steinar; Strømholt, Jo; Elvevoll, Edel O.; Eilertsen, Karl-Erik; Heimstad, Eldbjørg Sofie; Giæver, Ingeborg Hulda; Israelsen, Linn; Svenning, Jon Brage; Dalheim, Lars; Osvik, Renate Døving; Hansen, Espen Holst; Ingebrigtsen, Richard Andre; Aspen, Terje M; Wintervoll, Geir-Henning (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-03-17)
      From 2015 to 2021, we optimized mass cultivation of diatoms in our own developed vertical column airlift photobioreactors using natural and artificial light (LEDs). The project took place at the ferrosilicon producer Finnfjord AS in North Norway as a joint venture with UiT—The Arctic University of Norway. Small (0.1–6–14 m<sup>3</sup>) reactors were used for initial experiments and to produce inoculum ...
    • Mikroalger i fôr, ny løsning for å redusere lusepåslag på laks – Faglig sluttrapport 

      Hustad, Anette; Vang, Birthe; Eilertsen, Hans Christian; Siikavuopio, Sten Ivar; Sæther, Bjørn-Steinar; Svalheim, Ragnhild Aven; Hansen, Espen Holst; Svenning, Jon Brage; Kousoulaki, Katerina; Whitaker, Ragnhild (Research report; Forskningsrapport, 2020-05)
      I dette prosjektet har vi testet ut en hypotese om at ved å erstatte vegetabilske oljer i fôr til oppdrettslaks med marine oljer, vil man kunne redusere lusepåslag hos laks. Vi har fôret laks med fire ulike fôr, der proteininnholdet er det samme, men oljen er variert. De ulike fôrene vi brukte var kontrollfôr som var standardfôr med vegetabilsk olje, og tre ulike testfôr der vegetabilsk olje ble ...
    • Stability of lipids during wet storage of the marine diatom Porosira glacialis under semi-preserved conditions at 4 and 20 °C 

      Dalheim, Lars; Svenning, Jon Brage; Vasskog, Terje; Eilertsen, Hans Christian; Olsen, Ragnar Ludvig (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-10-17)
      Cultivation of diatoms may help alleviate the pressure on wild fish stocks for marine nutrient availability in aquaculture feed and for human consumption. However, the lipids in microalgae biomass are easily deteriorated, both trough lipolysis and degradation of polyunsaturated fatty acids (PUFA). Proper storage conditions are therefore necessary to maintain the lipid quality. Additionally, the ...
    • Structural and functional analyses of Rubisco from arctic diatom species reveal unusual posttranslational modifications 

      Valegård, Karin; Andralojc, P. John; Haslam, Richard P.; Pearce, F. Grant; Eriksen, Gunilla Kristina; Madgwick, Pippa J.; Kristoffersen, Anne Karin; van Lun, Michiel; Klein, Uwe; Eilertsen, Hans Christian; Parry, Martin A.J.; Andersson, Inger (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-06-20)
      The catalytic performance of the major CO<sub>2</sub>-assimilating enzyme, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), restricts photosynthetic productivity. Natural diversity in the catalytic properties of Rubisco indicates possibilities for improvement. Oceanic phytoplankton contain some of the most efficient Rubisco enzymes, and diatoms in particular are responsible for a significant ...
    • Temperature dependent growth rate, lipid content and fatty acid composition of the marine cold-water diatom Porosira glacialis 

      Svenning, Jon Brage; Dalheim, Lars; Eilertsen, Hans Christian; Vasskog, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-11-27)
      In this study, the northern cold-water marine diatom <i>Porosira glacialis</i> was cultivated in a pilot-scale mass cultivation system at 5 different temperatures (−2 to 12 °C), in order to evaluate temperature-dependent growth rate (in vitro Chl <i>a</i>), lipid content (Folch's method) and fatty acid (FA) composition (GC–MS) in the exponential growth phase. We found that <i>P. glacialis</i> has a ...
    • Two Isomeric C16 Oxo-Fatty Acids from the Diatom Chaetoceros karianus Show Dual Agonist Activity towards Human Peroxisome Proliferator-Activated Receptors (PPARs) α/γ 

      Moldes-Anaya, Angel; Sæther, Thomas; Uhlig, Silvio; Nebb, Hilde Irene; Larsen, Terje; Eilertsen, Hans Christian; Paulsen, Steinar Martin (Journal article; Tidsskriftartikkel; Peer reviewed, 2017-05-25)
      The peroxisome proliferator-activated receptors (PPARs) function as ligand-activated transcription factors that convert signals in the form of lipids to physiological responses through the activation of metabolic target genes. Due to their key roles in lipid and carbohydrate metabolism, the PPARs are important drug targets. However, for several of the PPAR drugs currently in use, adverse side effects ...