• Environmental DNA metabarcoding as an effective and rapid tool for fish monitoring in canals 

      McDevitt, Allan D; Sales, Naiara Guimaraes; Browett, Samuel S.; Sparnenn, Abbie O.; Mariani, Stefano; Wangensteen Fuentes, Owen Simon; Coscia, Ilaria; Benvenuto, Chiara (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-06-10)
      We focus on a case study along an English canal comparing environmental DNA (eDNA) metabarcoding with two types of electrofishing techniques (wade‐and‐reach and boom‐boat). In addition to corroborating data obtained by electrofishing, eDNA provided a wider snapshot of fish assemblages. Given the semi‐lotic nature of canals, we encourage the use of eDNA as a fast and cost‐effective tool to detect and ...
    • Fishing for mammals: Landscape‐level monitoring of terrestrial and semi‐aquatic communities using eDNA from riverine systems 

      Sales, Naiara Guimaraes; McKenzie, Maisie B.; Drake, Joseph; Harper, Lynsey R.; Browett, Samuel S.; Coscia, Ilaria; Wangensteen Fuentes, Owen Simon; Baillie, Charles; Bryce, Emma; Dawson, Deborah A.; Ochu, Erinma; Hänfling, Bernd; Lawson Handley, Lori; Mariani, Stefano; Lambin, Xavier; Sutherland, Christopher; McDevitt, Allan D (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-03-10)
      1: Environmental DNA (eDNA) metabarcoding has revolutionized biomonitoring in both marine and freshwater ecosystems. However, for semi‐aquatic and terrestrial animals, the application of this technique remains relatively untested.<p> <p>2: We first assess the efficiency of eDNA metabarcoding in detecting semi‐aquatic and terrestrial mammals in natural lotic ecosystems in the UK by comparing ...
    • Influence of preservation methods, sample medium and sampling time on eDNA recovery in a neotropical river 

      Sales, Naiara Guimaraes; Wangensteen Fuentes, Owen Simon; Carvalho, Daniel C.; Mariani, Stefano (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-05-31)
      Environmental DNA (eDNA) has rapidly emerged as a promising biodiversity monitoring technique, proving to be a sensitive and cost‐effective method for species detection. Despite the increasing popularity of eDNA, several questions regarding its limitations remain to be addressed. We investigated the effect of sampling medium and time, and preservation methods, on fish detection performance based on ...
    • Space-time dynamics in monitoring neotropical fish communities using eDNA metabarcoding 

      Sales, Naiara Guimaraes; Wangensteen Fuentes, Owen Simon; Carvalho, Daniel C.; Deiner, Kristy; Præbel, Kim; Coscia, Ilaria; McDevitt, Allan D; Mariani, Stefano (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-09-01)
      The biodiverse Neotropical ecoregion remains insufficiently assessed, poorly managed, and threatened by unregulated human activities. Novel, rapid and cost-effective DNA-based approaches are valuable to improve understanding of the biological communities and for biomonitoring in remote areas. Here, we evaluate the potential of environmental DNA (eDNA) metabarcoding for assessing the structure and ...