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dc.contributor.advisorŠkalko-Basnet, Nataša
dc.contributor.advisorStensrud, Gry
dc.contributor.authorKangu, Lloyd Mbugua
dc.date.accessioned2018-05-22T12:38:45Z
dc.date.available2018-05-22T12:38:45Z
dc.date.issued2016-05-12
dc.description.abstractChronic skin wounds are an exponentially increasing problem on a world basis, and are often hard to treat due to bacterial biofilm formation and resistance to different antibiotic treatments. Antimicrobial photodynamic therapy (aDPT) shows promise in resolving this issue, but clinical based treatments have yet to be established although photodynamic therapy is used for other conditions. A new chemical entity (NCE) encapsulated in chitosan/lecithin nanoparticles as well as NCE as a free drug was evaluated for potential cytotoxic effects by an in vitro MTT assay performed on HaCaT cells. The nanoparticles were varying in mean size (173.9±69.2 to 486.3±197.9) and zeta potential (16.5±1.67 to 66.3±1.15); depending on the chitosan/lecithin ratio, and the entrapment was between 42.1±0.3 to 84.4±0.2 %. To evaluate the cytotoxic effect different solutions of free NCE (50 to 500 mg/mL) and NCE in the nanoparticles (103.1 to 824.8 μg/mL) were tested. The results found non-significant decreases in cell viability for the lower concentrations, as well as significant increases in cell viability higher concentrations. Although NCE showed promising results with little to no toxicity in both formulations, further testing needs to be conducted before a final toxicity assessment can be made.en_US
dc.identifier.urihttps://hdl.handle.net/10037/12751
dc.language.isoengen_US
dc.publisherUiT Norges arktiske universiteten_US
dc.publisherUiT The Arctic University of Norwayen_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2016 The Author(s)
dc.subject.courseIDFAR-3911
dc.subjectMTT assayen_US
dc.subjectHaCaT cellsen_US
dc.subjectphotodynamic therapyen_US
dc.subjectchronic woundsen_US
dc.subjectdrug delivery systemen_US
dc.subjectnanoparticlesen_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Farmakologi: 728en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Pharmacology: 728en_US
dc.titleChitosan Lecithin Nanoparticles with New Chemical Entity. Nanotoxicity Evaluationen_US
dc.typeMaster thesisen_US
dc.typeMastergradsoppgaveen_US


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