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  • A comparative genomic and phenotypic study of Vibrio cholerae model strains using hybrid sequencing 

    Lorentzen, Øyvind Myrvoll; Bleis, Christina; Abel, Sören (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-08-23)
    Next-generation sequencing methods have become essential for studying bacterial biology and pathogenesis, often depending on high-quality, closed genomes. In this study, we utilized a hybrid sequencing approach to assemble the genome of C6706, a widely used Vibrio cholerae model strain. We present a manually curated annotation of the genome, enhancing user accessibility by linking each coding sequence ...
  • Validation of two LC–HRMS methods for large-scale untargeted metabolomics of serum samples: Strategy to establish method fitness-for-purpose 

    Grijseels, Sietske; Vasskog, Terje; Heinsvig, Pia J.; Myhre, Torbjørn Norberg; Hansen, Terkel; Mardal, Marie (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-08-06)
    Untargeted metabolomics by LCsingle bondHRMS is a powerful tool to enhance our knowledge of pathophysiological processes. Whereas validation of a bioanalytical method is customary in most analytical chemistry fields, it is rarely performed for untargeted metabolomics. This study aimed to establish and validate an analytical platform for a long-term, clinical metabolomics study. Sample preparation ...
  • Antimicrobial liposomes-in-nanofiber wound dressings prepared by a green and sustainable wire-electrospinning set-up 

    Schulte-Werning, Laura Victoria; Singh, Bhupender; Johannessen, Mona Susan; Engstad, Rolf Einar; Holsæter, Ann-Mari (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-04-19)
    Increasing prevalence of infected and chronic wounds demands improved therapy options. In this work an electrospun nanofiber dressing with liposomes is suggested, focusing on the dressing’s ability to support tissue regeneration and infection control. Chloramphenicol (CAM) was the chosen antibiotic, added to the nanofibers after first embedded in liposomes to maintain a sustained drug release. ...
  • Epistasis arises from shifting the rate-limiting step during enzyme evolution of a β-lactamase 

    Frøhlich, Christopher; Bunzel, H. Adrian; Buda, Karol; Mulholland, Adrian J.; van der Kamp, Marc W.; Johnsen, Pål Jarle; Leiros, Hanna-Kirsti S.; Tokuriki, Nobuhiko (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-02-23)
    Epistasis, the non-additive efect of mutations, can provide combinatorial improvements to enzyme activity that substantially exceed the gains from individual mutations. Yet the molecular mechanisms of epistasis remain elusive, undermining our ability to predict pathogen evolution and engineer biocatalysts. Here we reveal how directed evolution of a β-lactamase yielded highly epistatic activity ...
  • Barriers and facilitators of pharmacists’ integration in a multidisciplinary home care team: a qualitative interview study based on the normalization process theory 

    Bø, Karl-Erik; Halvorsen, Kjell Hermann; Le, Yen-Ngoc Anna; Lehnbom, Elin Christina (Journal article; Tidsskriftartikkel, 2024-05-02)
    <p><i>Background</i> There is a growing recognition of multidisciplinary practices as the most rational approach to providing better and more efficient healthcare services. Pharmacists are increasingly integrated into primary care teams, but there is no universal approach to implementing pharmacist services across healthcare settings. In Norway, most pharmacists work in pharmacies, with very few ...
  • Chitosan and liposomal delivery systems for epicatechin or propyl gallate targeting localized treatment of vulvovaginal candidiasis 

    Mork, Silje; Johannessen, Mona Susan; Skalko Basnet, Natasa; Jøraholmen, May Wenche Bakke (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-07-18)
    Natural polyphenols are promising alternatives to antifungals for novel treatments of vulvovaginal candidiasis (VVC) in an era of antimicrobial resistance. However, polyphenols are poorly soluble and prone to degradation. To overcome their limitations, we propose incorporation in liposomes. The study aimed to develop chitosan and liposome comprising delivery systems for epicatechin (EC) or propyl ...
  • Urinary excretion profile of higenamine in females after oral administration of supplements – Doping scenario 

    Stojanovic, B.; Rasic, J.; Andjelkovic, M.; Dikic, N.; Dragicevic, N.; Djordjevic, B.; Forsdahl, Guro; Gmeiner, G. (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-02-15)
    In 2017, higenamine was added to the World Antidoping Agency’s (WADA) Prohibited list under group S3: beta-2 agonists and it is banned for athletes both in – and out of competition. Aim of this study was to characterize the urinary excretion profile of higenamine and its metabolite coclaurine after oral administration of multiple doses of higenamine capsules. For this purpose, an administration ...
  • The recombination initiation functions DprA and RecFOR suppress microindel mutations in Acinetobacter baylyi ADP1 

    Liljegren, Mikkel Meyn; Gama, João Alves; Johnsen, Pål Jarle; Harms, Klaus (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-05-17)
    Short-Patch Double Illegitimate Recombination (SPDIR) has been recently identified as a rare mutation mechanism. During SPDIR, ectopic DNA single-strands anneal with genomic DNA at microhomologies and get integrated during DNA replication, presumably acting as primers for Okazaki fragments. The resulting microindel mutations are highly variable in size and sequence. In the soil bacterium Acinetobacter ...
  • Health-related quality of life among older adults following acute hospitalization: longitudinal analysis of a randomized controlled trial 

    Robinson, Eirin Guldsten; Gyllensten, Hanna; Granås, Anne Gerd; Halvorsen, Kjell Hermann; Garcia, Beate Hennie (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-06-17)
    Purpose To describe the longitudinal change of health-related quality of life (HRQoL) over 12 months from acute hospitalization in older adults≥70 years (IMMENSE study), and associated factors, to investigate how a medication optimization intervention infuenced this change.<p> <p>Methods The EQ-5D-3L was used at discharge and 1, 6 and 12 months after discharge during a randomized controlled trial ...
  • Methylation of phase II metabolites of endogenous anabolic androgenic steroids to improve analytical performance 

    Pfeffer, Sandra; Gmeiner, Guenter; Forsdahl, Guro (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-04-21)
    The study of intact phase II metabolites of endogenous anabolic androgenic steroids (EAAS) gives important information about metabolism and has the potential to improve the detection of doping with testosterone. For analysis with liquid chromatography–mass spectrometry (LC-MS), chemical derivatization at the steroid moiety is a technique to improve the positive ionization efficiency of glucuronida ...
  • Plasma tryptophan pathway metabolites quantified by liquid chromatography-tandem mass spectrometry as biomarkers in neuroendocrine tumor patients 

    Johansen, Silje Udjus; Hansen, Terkel; Nordborg, Anna; Meyer, Renate Weenås; Goll, Rasmus; Florholmen, Jon; Jensen, Einar (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-02-15)
    A good and accessible biomarker is of great clinical value in neuroendocrine tumor (NET) patients, especially considering its frequently indolent nature and long-term follow-up. Plasma chromogranin A (CgA) and 5-hydroxyindoleacetic acid (5-HIAA) are currently used as biomarkers in NET, but their sensitivity and specificity are restricted. 5-HIAA is the main metabolite of serotonin, an important ...
  • “Using network analysis modularity to group health code systems and decrease dimensionality in machine learning models” 

    Askar, Mohsen Gamal Saad; Småbrekke, Lars; Holsbø, Einar Jakobsen; Bongo, Lars Ailo Aslaksen; Svendsen, Kristian (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-06-11)
    Background: Machine learning (ML) prediction models in healthcare and pharmacy-related research face challenges with encoding high-dimensional Healthcare Coding Systems (HCSs) such as ICD, ATC, and DRG codes, given the trade-off between reducing model dimensionality and minimizing information loss. Objectives: To investigate using Network Analysis modularity as a method to group HCSs to improve ...
  • Dissemination of extensively drug-resistant NDM-producing Providencia stuartii in Europe linked to patients transferred from Ukraine, March 2022 to March 2023 

    Witteveen, Sandra; Hans, Jörg B; Izdebski, Radosław; Hasman, Henrik; Samuelsen, Ørjan; Dortet, Laurent; Pfeifer, Yvonne; Delappe, Niall; Oteo-Iglesias, Jesús; Żabicka, Dorota; Cormican, Martin; Sandfort, Mirco; Reichert, Felix; Pöntinen, Anna Kaarina; Fischer, Martin A.; Verkaik, Nelianne; Pérez-Vazquez, María; Pfennigwerth, Niels; Hammerum, Anette M.; Hallstrøm, Søren; Biedrzycka, Marta; Räisänen, Kati; Wielders, Cornelia Ch; Urbanowicz, Paweł; de Haan, Angela; Westmo, Karin; Landman, Fabian; van der Heide, Han Gj; Lansu, Simon; Zwittink, Romy D.; Notermans, Daan W.; Guzek, Aneta; Kondratiuk, Viacheslav; Salmanov, Aidyn; Haller, Sebastian; Linkevicius, Marius; Gatermann, Sören; Kohlenberg, Anke; Gniadkowski, Marek; Werner, Guido; Hendrickx, Antoni Pa (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-06-06)
    Background: The war in Ukraine led to migration of Ukrainian people. Early 2022, several European national surveillance systems detected multidrugresistant (MDR) bacteria related to Ukrainian patients.<p> <p>Aim: To investigate the genomic epidemiology of New Delhi metallo-β-lactamase (NDM)-producing Providencia stuartii from Ukrainian patients among European countries. <p>Methods: Whole-genome ...
  • Exploring the impact of pharmacist-supported medication reviews in dementia care: experiences of general practitioners and nurses 

    Carlqvist, Catharina; Ekstedt, Mirjam; Lehnbom, Elin Christina (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-06-14)
    Background Dementia is a major global public health challenge, and with the growing elderly population, its prevalence is expected to increase in the coming years. In Sweden, municipalities are responsible for providing special housing for the elderly (SÄBO), which offers services and care for older individuals needing specific support. SÄBO is both the person´s home and a care environment and ...
  • Deep sequencing of Escherichia coli exposes colonisation diversity and impact of antibiotics in Punjab, Pakistan 

    Khawaja, Tamim; Mäklin, Tommi; Kallonen, Teemu; Gladstone, Rebecca Ashley; Pöntinen, Anna Kaarina; Mero, Sointu; Thorpe, Harry A.; Samuelsen, Ørjan; Parkhill, Julian; Izhar, Mateen; Akhtar, M. Waheed; Corander, Jukka; Kantele, Anu (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-06-18)
    Multi-drug resistant (MDR) E. coli constitute a major public health burden globally, reaching the highest prevalence in the global south yet frequently flowing with travellers to other regions. However, our comprehension of the entire genetic diversity of E. coli colonising local populations remains limited. We quantified this diversity, its associated antimicrobial resistance (AMR), and assessed ...
  • Lipidome Plasticity Enables Unusual Photosynthetic Flexibility in Arctic vs. Temperate Diatoms 

    Svenning, Jon Brage; Vasskog, Terje; Campbell, Karley Lynn; Bæverud, Agnethe Hansen; Myhre, Torbjørn Norberg; Dalheim, Lars; Forgereau, Zoé Lulu; Osanen, Janina Emilia; Hansen, Espen Holst; Bernstein, Hans Christopher (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-01-27)
    The diatom lipidome actively regulates photosynthesis and displays a high degree of plasticity in response to a light environment, either directly as structural modifications of thylakoid membranes and protein–pigment complexes, or indirectly via photoprotection mechanisms that dissipate excess light energy. This acclimation is crucial to maintaining primary production in marine systems, particularly ...
  • The Biofilm Lifestyle Shapes the Evolution of β-Lactamases 

    Lorentzen, Øyvind Myrvoll; Haukefer, Anne Sofie B.; Johnsen, Pål Jarle; Frøhlich, Christopher (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-02-15)
    The evolutionary relationship between the biofilm lifestyle and antibiotic resistance enzymes remains a subject of limited understanding. Here, we investigate how β-lactamases affect biofilm formation in Vibrio cholerae and how selection for a biofilm lifestyle impacts the evolution of these enzymes. Genetically diverse β-lactamases expressed in V. cholerae displayed a strong inhibitory effect on ...
  • Using real world data for pharmacoeconomic assessments - a case study 

    Haukaas, Jeroen Martin van Zuiden (Mastergradsoppgave; Master thesis, 2024-05-14)
    Pembrolizumab monotherapy for non-small cell lung cancer (NSCLC) was deemed not cost-effective due to limited data, high uncertainty regarding long term results and a considerable budget impact. However, due to confidential price agreements it was approved in 2017, as first-line treatment for metastatic NSCLC that expresses PD-L1 ≥ 50 %. Given potential discrepancies between clinical efficacy and ...
  • Can a mock medication-taking learning activity enable pharmacy students to experience the range of barriers and facilitators to medication adherence? An analysis informed by the Theoretical Domains Framework and COM-B model 

    Mantzourani, E.; James, D.H.; Akthar, M.A.; Brown, S.L.; Yemm, R.; Lehnbom, Elin Christina; Hanrahan, J.R.; Seage, C.H. (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-12-08)
    Background: Pharmacy professionals are well-placed to provide medication adherence support to patients. The Capability, Opportunity, Motivation-Behaviour (COM–B) and Theoretical Domains Framework (TDF) are two complementary models previously applied to medication-taking behaviour. Understanding the patient-specific barriers and facilitators to adherence using psychological frameworks from the ...
  • Modulation of multidrug-resistant clone success in Escherichia coli populations: a longitudinal, multi-country, genomic and antibiotic usage cohort study 

    Pöntinen, Anna Kaarina; Gladstone, Rebecca Ashley; Pesonen, Henri; Pesonen, Maiju; Cleon, Francois Pierre Alexandre; Parcell, Benjamin J; Kallonen, Teemu; Simonsen, Gunnar Skov; Croucher, Nicholas J; McNally, Alan; Parkhill, Julian; Johnsen, Pål Jarle; Samuelsen, Ørjan; Corander, Jukka (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-01-11)
    Background The effect of antibiotic usage on the success of multidrug-resistant (MDR) clones in a population remains unclear. With this genomics-based molecular epidemiology study, we aimed to investigate the contribution of antibiotic use to Escherichia coli clone success, relative to intra-strain competition for colonisation and infection.<p> <p>Methods We sequenced all the available E coli ...

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