Recent additions

  • Current challenges and future perspectives in oral absorption research: An opinion of the UNGAP network 

    Vinarov, Zahari; Abrahamsson, Bertil; Artursson, Per; Batchelor, Hannah; Berben, Philippe; Bernkop-Schnürch, Andreas; Butler, James; Ceulemans, Jens; Davies, Nigel; Dupont, Didier; Flaten, Gøril Eide; Fotaki, Nikoletta; Griffin, Brendan T.; Jannin, Vincent; Keemink, Janneke; Kesisoglou, Filippos; Koziolek, Mirko; Kuentz, Martin; Mackie, Alan R.; Meléndez-Martínez, Antonio J.; McAllister, Mark; Müllertz, Anette; O'Driscoll, Caitriona M.; Parrott, Neil; Paszkowska, Jadwiga; Pavek, Petr; Porter, Christopher J.H.; Reppas, Christos; Stillhart, Cordula; Sugano, Kiyohiko; Toader, Elena; Valentová, Kateřina; Vertzoni, Maria; De Wildt, Saskia N.; Wilson, Clive G.; Augustijns, Patrick (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-02-18)
    Although oral drug delivery is the preferred administration route and has been used for centuries, modern drug discovery and development pipelines challenge conventional formulation approaches and highlight the insufficient mechanistic understanding of processes critical to oral drug absorption. This review presents the opinion of UNGAP scientists on four key themes across the oral absorption ...
  • High-Intensity Exercise Improves Fatigue, Sleep, and Mood in Patients With Axial Spondyloarthritis: Secondary Analysis of a Randomized Controlled Trial 

    Sveaas, Silje Halvorsen; Dagfinrud, Hanne; Berg, Inger Jorid; Provan, Sella Aarrestad; Johansen, Melissa Woll; Pedersen, Elisabeth; Bilberg, Annelie (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-05-04)
    <i>Objective</i> - Although exercise is recommended in the treatment of axial spondyloarthritis (axSpa), the focus has been on flexibility, and the effect of high-intensity exercises is unknown. The purpose of this study was to investigate the effect of high-intensity exercises on fatigue, sleep, and mood in patients with axSpA.<br><br> <i>Methods</i> - In this secondary analysis of a randomized ...
  • Dyes in Disguise: characterization of fluorescently labeled liposomes 

    Cauzzo, Jennifer; Nystad, Mona; Holsæter, Ann Mari; Basnet, Purusotam; Skalko-Basnet, Natasa (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-07-09)
    The rather limited success of translation from basic research to clinical application has been highlighted as a major issue in the nanomedicine field. To identify the factors influencing the applicability of nanosystems as drug carriers and potential nanomedicine, we focused on following their fate through fluorescence-based assays, namely flow cytometry and imaging. These methods are often used to ...
  • Predicting oral absorption of fenofibrate in lipid-based drug delivery systems by combining in vitro lipolysis with the mucus-PVPA permeability model 

    Falavigna, Margherita; Klitgaard, Mette; Berthelsen, Ragna; Mullertz, Anette; Flaten, Gøril Eide (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-09-08)
    The aim of this work was to develop a new in vitro lipolysis-permeation model to predict the in vivo absorption of fenofibrate in self-nanoemulsifying drug delivery systems (SNEDDSs). More specifically, the in vitro intestinal lipolysis model was combined with the mucus-PVPA (Phospholipid Vesicle-based Permeation Assay) in vitro permeability model. Biosimilar mucus (BM) was added to the surface of ...
  • Tn1 transposition in the course of natural transformation enables horizontal antibiotic resistance spread in Acinetobacter baylyi 

    Kloos, Julia Maria; Johnsen, Pål Jarle; Harms, Klaus (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-12-03)
    Transposons are genetic elements that change their intracellular genomic position by transposition and are spread horizontally between bacteria when located on plasmids. It was recently discovered that transposition from fully heterologous DNA also occurs in the course of natural transformation. Here, we characterize the molecular details and constraints of this process using the replicative transposon ...
  • Barrierer mot etterlevelse av retningslinjer for helsepersonell i sykehus 

    Evenstad, Berit; Larsen, Lill Sverresdatter; Gravningen, Kirsten Midttun (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-10-28)
    <p><i>Bakgrunn:</i> Kunnskapsbaserte faglige retningslinjer skal bidra til å kvalitetssikre helsetjenesten. Tidligere forskning viser et gap mellom anbefalinger i retningslinjer og klinisk praksis. Få studier har undersøkt etterlevelsen av retningslinjer blant helsepersonell på sykehus etter avsluttet implementering. Denne studien tar utgangspunkt i «Keisersnittprosjektet», et kvalitetsforbedringsprosjekt ...
  • Biomimicry of microbial polysaccharide hydrogels for tissue engineering and regenerative medicine - A review 

    Ng, Jian Yao; Obuobi, Sybil Akua Okyerewa; Chua, Mei Ling; Zhang, Chi; Hong, Shiqi; Kumar, Yogesh; Gokhale, Rajeev; Ee, Pui Lai Rachel (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-04-29)
    Hydrogels as artificial biomaterial scaffolds offer a much favoured 3D microenvironment for tissue engineering and regenerative medicine (TERM). Towards biomimicry of the native ECM, polysaccharides from Nature have been proposed as ideal surrogates given their biocompatibility. In particular, derivatives from microbial sources have emerged as economical and sustainable biomaterials due to their ...
  • Liposomes-in-hydrogel delivery system enhances the potential of resveratrol in combating vaginal chlamydia infection 

    Jøraholmen, May Wenche; Johannessen, Mona; Gravningen, Kirsten Midttun; Puolakkainen, Mirja; Acharya, Ganesh; Basnet, Purusotam; Skalko-Basnet, Natasa (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-12-11)
    <i>Chlamydia trachomatis</i> is the most common cause of bacterial sexually transmitted infections and causes serious reproductive tract complications among women. The limitations of existing oral antibiotics and treatment of antimicrobial resistance require alternative treatment options. We are proposing, for the first time, the natural polyphenol resveratrol (RES) in an advanced delivery system ...
  • Liposomal delivery of antibiotic loaded nucleic acid nanogels with enhanced drug loading and synergistic anti-inflammatory activity against S. aureus intracellular infections 

    Obuobi, Sybil Akua Okyerewa; Julin, Kjersti; Fredheim, Elizabeth G. Aarag; Johannessen, Mona; Skalko-Basnet, Natasa (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-07)
    The persistence of <i>Staphylococcus aureus</i> has been accredited to its ability to escape immune response via host cell invasion. Despite the efficacy of many antibiotics against <i>S. aureus</i>, the high extracellular concentrations of conventional antibiotics required for bactericidal activity is limited by their low cellular accumulation and poor intracellular retention. While nanocarriers ...
  • Simultaneous assessment of in vitro lipolysis and permeation in the mucus-PVPA model to predict oral absorption of a poorly water soluble drug in SNEDDSs 

    Falavigna, Margherita; Brurok, Sunniva; Klitgaard, Mette; Flaten, Gøril Eide (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-01-21)
    The prediction of the <i>in vivo</i> performance of self-nanoemulsifying drug delivery systems (SNEDDSs) is currently gaining increasing attention. Therefore, the need for reliable <i>in vitro</i> models able to assess the drug solubilization capacity of such formulations upon <i>in vitro</i> lipolysis, as well as to concomitantly evaluate <i>in vitro</i> drug permeation, has become ever so evident. ...
  • Cross-border spread of blaNDM-1- and blaOXA-48-positive Klebsiella pneumoniae: a European collaborative analysis of whole genome sequencing and epidemiological data, 2014 to 2019 

    Ludden, Catherine; Lötsch, Felix; Alm, Erik; Kumar, Narender; Johansson, Karin; Albiger, Barbara; Huang, Te-Din; Denis, Olivier; Hammerum, Anette M.; Hasman, Henrik; Jalava, Jari; Räisänen, Kati; Dortet, Laurent; Jousset, Agnès B.; Gatermann, Sören; Haller, Sebastian; Cormican, Martin; Brennan, Wendy; Grosso, Maria Del; Monaco, Monica; Schouls, Leo; Samuelsen, Ørjan; Pirš, Mateja; Cerar, Tjaša; Oteo-Iglesias, Jésus; Pérez-Vázquez, Maria; Sjöström, Karin; Edquist, Petra; Hopkins, Katie L.; Struelens, Marc J.; Palm, Daniel; Monnet, Dominique L.; Kohlenberg, Anke (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-05-21)
    An alert regarding an outbreak of carbapenem-resistant <i>Klebsiella pneumoniae</i> carrying <i>bla</i> <sub>NDM-1</sub> and <i>bla</i> <sub>OXA-48</sub> carbapenemase-encoding genes was sent by Germany to European Union (EU)/European Economic Area (EEA) countries in October 2019. Since only limited whole genome sequencing (WGS) data on <i>bla</i> <sub>NDM-1-</sub> and <i>bla</i> <sub>OXA-48</sub>-positive ...
  • Challenges and Best Practices in Ethical Review of Human and Organizational Factors Studies in Health Technology: a Synthesis of Testimonies 

    Peute, Linda W.; Lichtner, Valentina; Baysari, Melissa T.; Hägglund, Maria; Homco, Juell; Jansen-Kosterink, Stephanie; Jauregui, Ignacio; Kaipio, Johanna; Kuziemsky, Craig E.; Lehnbom, Elin; Leite, Francisca; Lesselroth, Blake; Luna, Daniel; Otero, Carlos; Pedersen, Rune; Pelayo, Sylvia; Santos, Raquel; Silva, Nuno-André; Tyllinen, Mari; Van Velsen, Lex; Zheng, Wu Yi; Jaspers, Monique; Marcilly, Romaric (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-04-17)
    <i>Objective</i>: Human and Organizational Factors (HOF) studies in health technology involve human beings and thus require Institutional Review Board (IRB) approval. Yet HOF studies have specific constraints and methods that may not fit standard regulations and IRB practices. Gaining IRB approval may pose difficulties for HOF researchers. This paper aims to provide a first overview of HOF study ...
  • Genetic dominance governs the evolution and spread of mobile genetic elements in bacteria 

    Rodríguez-Beltrán, Jerónimo; Sørum, Vidar; Toll-Riera, Macarena; de la Vega, Carmen; Peña-Miller, Rafael; San Millán, Álvaro (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-22)
    Mobile genetic elements (MGEs), such as plasmids, promote bacterial evolution through horizontal gene transfer (HGT). However, the rules governing the repertoire of traits encoded on MGEs remain unclear. In this study, we uncovered the central role of genetic dominance shaping genetic cargo in MGEs, using antibiotic resistance as a model system. MGEs are typically present in more than one copy per ...
  • Selection or drift: The population biology underlying transposon insertion sequencing experiments 

    Mahmutovic, Anel; Abel zur Wiesch, Pia; Abel, Sören (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-03-25)
    <p>Transposon insertion sequencing methods such as Tn-seq revolutionized microbiology by allowing the identification of genomic loci that are critical for viability in a specific environment on a genome-wide scale. While powerful, transposon insertion sequencing suffers from limited reproducibility when different analysis methods are compared. From the perspective of population biology, this may be ...
  • All non-adherence is equal, but is some more equal than others? TB in the digital era 

    Stagg, Helen; Flook, Mary; Martinecz, Antal; Kielmann, Karina; Abel zur Wiesch, Pia; Karat, Aaron; Lipman, Marc; Sloan, Derek; Walker, Elizabeth; Fielding, Katherine L (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-11-02)
    Adherence to treatment for tuberculosis (TB) has been a concern for many decades, resulting in the World Health Organization's recommendation of the direct observation of treatment in the 1990s. Recent advances in digital adherence technologies (DATs) have renewed discussion on how to best address nonadherence, as well as offering important information on dose-by-dose adherence patterns and their ...
  • Phosphorylation of steroid receptor coactivator-3 (SRC-3) at serine 857 is regulated by the p38 MAPK-MK2 axis and affects NF-κB-mediated transcription 

    Shrestha, Anup; Bruckmüller, Henrike; Kildalsen, Hanne; Kaur, Gurjit; Gaestel, Matthias; Ljones Wetting, Hilde; Mikkola, Ingvild; Seternes, Ole Morten (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-09)
    Steroid receptor coactivator-3 (SRC-3) regulates the activity of both nuclear hormone receptors and a number of key transcription factors. It is implicated in the regulation of cell proliferation, inflammation and in the progression of several common cancers including breast, colorectal and lung tumors. Phosphorylation is an important regulatory event controlling the activities of SRC-3. Serine 857 ...
  • Drug-target binding quantitatively predicts optimal antibiotic dose levels in quinolones 

    Clarelli, Fabrizio; Palmer, Adam; Singh, Bhupender; Storflor, Merete; Lauksund, Silje; Cohen, Ted; Abel, Sören; Abel zur Wiesch, Pia (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-08-14)
    Antibiotic resistance is rising and we urgently need to gain a better quantitative understanding of how antibiotics act, which in turn would also speed up the development of new antibiotics. Here, we describe a computational model (COMBAT-COmputational Model of Bacterial Antibiotic Target-binding) that can quantitatively predict antibiotic dose-response relationships. Our goal is dual: We address a ...
  • Plasmid Interactions Can Improve Plasmid Persistence in Bacterial Populations 

    Gama, João Alves; Zilhão, Rita; Dionisio, Francisco (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-08-31)
    It is difficult to understand plasmid maintenance in the absence of selection and theoretical models predict the conditions for plasmid persistence to be limited. Plasmid-associated fitness costs decrease bacterial competitivity, while imperfect partition allows the emergence of plasmid-free cells during cell division. Although plasmid conjugative transfer allows mobility into plasmid-free cells, ...
  • Multi-scale modeling of drug binding kinetics to predict drug efficacy 

    Clarelli, Fabrizio; Liang, Jingyi; Martinecz, Antal; Heiland, Ines; Wiesch, Pia Abel Zur (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-11-25)
    Optimizing drug therapies for any disease requires a solid understanding of pharmacokinetics (the drug concentration at a given time point in different body compartments) and pharmacodynamics (the effect a drug has at a given concentration). Mathematical models are frequently used to infer drug concentrations over time based on infrequent sampling and/or in inaccessible body compartments. Models are ...
  • Plasmid Interactions Can Improve Plasmid Persistence in Bacterial Populations 

    Gama, João Alves; Zilhão, Rita; Dionisio, Francisco (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-08-31)
    It is difficult to understand plasmid maintenance in the absence of selection and theoretical models predict the conditions for plasmid persistence to be limited. Plasmid-associated fitness costs decrease bacterial competitivity, while imperfect partition allows the emergence of plasmid-free cells during cell division. Although plasmid conjugative transfer allows mobility into plasmid-free cells, ...

View more