Exploring the viability of using liposomes and bacterial extracellular vesicles (bEVs) in interaction studies by calorimetric methods
Permanent lenke
https://hdl.handle.net/10037/37048Dato
2024-05-14Type
MastergradsoppgaveMaster thesis
Forfatter
Braa, Brage HjelleSammendrag
The increasing level of AMR require an increase in new antimicrobial drug candidates. By
proving that bEVs are a suitable model for the bacterial membrane, it can be applied to the
development of new drug candidates. Potentially making the development of new candidates
more efficient and reducing cost.
To further examine the potential of bEVs, we isolated bEVs from E. coli, a gram-negative
bacteria. The bEVs were characterized using zeta-potential, size distribution, and protein
concentration measurements, while DMPC and DMPC-LPS liposomes were characterized
using zeta-potential and size distribution measurements. The differences of the
thermodynamic properties were compared using a differential scanning calorimetry (DSC)
and isothermal titration calorimetry (ITC) instrument. The consequences of the alterations
within the cell membrane of the bEVs caused by the different resistance mechanisms were
measured with the ITC instrument.
The analysis showed promising results regarding the thermodynamic properties and
differences caused by the resistance mechanisms. This study provides support to the claim
that bEVs are well suited to simulate the bacterial cell membrane. More extensive studies are
required to fully explore the potential of bEVs.
Forlag
UiT Norges arktiske universitetUiT The Arctic University of Norway
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