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dc.contributor.authorJamaly, Simin
dc.contributor.authorBasavaraj, Manjunath Goolyam
dc.contributor.authorStarikova, Irina
dc.contributor.authorOlsen, Randi
dc.contributor.authorBrækkan, Sigrid K.
dc.contributor.authorHansen, John-Bjarne
dc.date.accessioned2018-07-02T08:13:48Z
dc.date.available2018-07-02T08:13:48Z
dc.date.issued2018-05-31
dc.description.abstractBackground<br> Microvesicles (MVs) express antigens from their parental cells and have a highly procoagulant surface. Animal studies suggest that P‐Selectin Glycoprotein Ligand‐1 positive (PSGL‐1+) MVs play a role in the pathogenesis of venous thromboembolism (VTE).<p> Objective<br> The aim of this study was to determine plasma levels, the cellular origin and the morphological characteristics of PSGL‐1+ MVs in patients with unprovoked VTE.<p> Methods<br> We conducted a population based case‐control study in 20 patients with a history of unprovoked VTE and 20 age‐ and sex‐matched healthy controls recruited from the general population. Plasma levels, the cellular origin and the morphological characteristics of PSGL‐1+ MVs was evaluated using flow cytometry, electron microscopy and confocal microscopy.<p> Results<br> Plasma levels of PSGL‐1+ MVs were associated with increased risk of VTE. The odds ratio (OR) per one standard deviation (SD) increase in PSGL‐1+ MVs was 3.11 (95% CI: 1.41‐6.88) after adjustment for age and sex, and 2.88 (95% CI: 1.29‐6.41) after further adjustment for BMI. The PSGL‐1+ MVs originated mainly from monocytes and endothelial cells determined by double‐staining with markers of parental cells using flow cytometry and transmission electron microscopy (TEM). Scanning electron microscopy (SEM) of PSGL‐1 labeled plasma‐derived MVs displayed dominantly spherical vesicles that varied between 50 nm and 300 nm in diameter.<p> Conclusions<br> Increased plasma levels of PSGL‐1+ MVs are associated with the risk of unprovoked VTE. Large population‐based prospective studies are required to validate our findings.en_US
dc.description.sponsorshipStiftelsen K.G. Jebsenen_US
dc.description<a href=https://doi.org/10.1111/jth.14162> https://doi.org/10.1111/jth.14162 </a>.en_US
dc.identifier.citationJamaly, S., Basavaraj, M.G., Starikova, I., Olsen, R., Brækkan S. & Hansen, J.B. (2018). Elevated Plasma Levels of P‐Selectin Glycoprotein Ligand‐1 Positive Microvesicles in Patients with Unprovoked Venous Thromboembolism. Journal of Thrombosis and Haemostasis. https://doi.org/10.1111/jth.14162.en_US
dc.identifier.cristinIDFRIDAID 1588798
dc.identifier.issn1538-7933
dc.identifier.issn1538-7836
dc.identifier.urihttps://hdl.handle.net/10037/13087
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalJournal of Thrombosis and Haemostasis
dc.rights.accessRightsopenAccessen_US
dc.subjectCell-Derived Microparticlesen_US
dc.subjectP-Selectinen_US
dc.subjectVenous Thromboembolismen_US
dc.subjectPlateletsen_US
dc.subjectCase-Control Studiesen_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750en_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750en_US
dc.titleElevated Plasma Levels of P‐Selectin Glycoprotein Ligand‐1 Positive Microvesicles in Patients with Unprovoked Venous Thromboembolismen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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