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dc.contributor.authorUlvestad, Johanne Skår
dc.contributor.authorKumari, Jaya
dc.contributor.authorSeternes, Tore
dc.contributor.authorChi, Heng
dc.contributor.authorDalmo, Roy Ambli
dc.date.accessioned2018-08-21T12:12:14Z
dc.date.available2018-08-21T12:12:14Z
dc.date.issued2018-03-30
dc.description.abstractReactive oxygen species (ROS) production in macrophage‐like cells is induced as an antimicrobial defence against invading pathogens. In this study, we have explored how different stimuli and metabolic inhibitors affect the level of respiratory burst in Atlantic salmon (<i>Salmo salar</i> L.) head kidney macrophage‐like cells. Cells stimulated in vitro by bacterial lipopolysaccharide (LPS) and ß‐glucan showed increased production of ROS compared to unstimulated cells. Both stimulation and costimulation by curdlan (ß‐glucan) induced a higher production of ROS compared to stimulation and costimulation by LPS. Metabolic inhibitors co‐incubated with the stimulants did not, in most cases, perturb the level of ROS generation in the salmon macrophage‐like cells. The NAD<sup>+</sup> content as well as the NAD<sup>+</sup>/NADH ratio increased in curdlan and LPS + curdlan‐stimulated cells compared to control cells, which indicated increased metabolic activity in the stimulated cells. Supporting these findings, gene analysis using real‐time quantitative PCR showed that arginase‐1 and IL‐1ß genes were highly expressed in the stimulated cells.en_US
dc.description.sponsorshipTromsø Research Foundation UiT The Arctic University of Norwayen_US
dc.descriptionThis is the peer reviewed version of the following article: Ulvestad, J.S., Kumari, K., Seternes, T., Chi, H. & Dalmo, R.A. (2018). Studies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophages. Journal of Fish Diseases, 41(7), 1117-1127. https://doi.org/10.1111/jfd.12806, which has been published in final form at <a href=https://doi.org/10.1111/jfd.12806> https://doi.org/10.1111/jfd.12806</a>. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.en_US
dc.identifier.citationUlvestad, J.S., Kumari, K., Seternes, T., Chi, H. & Dalmo, R.A. (2018). Studies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophages. Journal of Fish Diseases, 41(7), 1117-1127. https://doi.org/10.1111/jfd.12806en_US
dc.identifier.cristinIDFRIDAID 1576941
dc.identifier.issn0140-7775
dc.identifier.issn1365-2761
dc.identifier.urihttps://hdl.handle.net/10037/13497
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalJournal of Fish Diseases
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/HAVBRUK2/237315/Norway/Fish Virus Vaccines/ViVaFish/en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/NANO2021/239140/Norway/Nanoparticle encapsulation of plant-based vaccine against piscine reovirus infection in salmon//en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920en_US
dc.subjectVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920en_US
dc.subjectgene expressionen_US
dc.subjectmacrophagesen_US
dc.subjectmetabolismen_US
dc.subjectrespiratory bursten_US
dc.subjectsalmonen_US
dc.titleStudies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophagesen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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