dc.contributor.author | Wang, Xumei | |
dc.contributor.author | Gusarova, Galina | |
dc.contributor.author | Ruhsam, Markus | |
dc.contributor.author | de Vere, Natasha | |
dc.contributor.author | Metherell, Chris | |
dc.contributor.author | Hollingsworth, Peter M. | |
dc.contributor.author | Twyford, Alex D. | |
dc.date.accessioned | 2019-02-20T10:43:31Z | |
dc.date.available | 2019-02-20T10:43:31Z | |
dc.date.issued | 2018-04-24 | |
dc.description.abstract | DNA barcoding is emerging as a useful tool not only for species identification but also for studying evolutionary and ecological processes. Although plant DNA barcodes do not always provide species-level resolution, the generation of large DNA barcode data sets can provide insights into the mechanisms underlying the generation of species diversity. Here, we study evolutionary processes in taxonomically complex British <i>Euphrasia</i> (Orobanchaceae), a group with multiple ploidy levels, frequent self-fertilization, young species divergence and widespread hybridization. We use a phylogenetic approach to investigate the colonization history of British <i>Euphrasia</i>, followed by a DNA barcoding survey and population genetic analyses to reveal the causes of shared sequence variation. Phylogenetic analysis shows <i>Euphrasia</i>have colonized Britain from mainland Europe on multiple occasions. DNA barcoding reveals that no British <i>Euphrasia</i>species has a consistent diagnostic sequence profile, and instead, plastid haplotypes are either widespread across species, or are population specific. The partitioning of nuclear genetic variation suggests differences in ploidy act as a barrier to gene exchange, while the divergence between diploid and tetraploid ITS sequences supports the polyploids being allotetraploid in origin. Overall, these results show that even when lacking species-level resolution, analyses of DNA barcoding data can reveal evolutionary patterns in taxonomically complex genera. | en_US |
dc.description.sponsorship | The Chinese Scholarship Council
The Scottish Government’s Rural and Environment Science and Analytical Services Division
Research Council of Norway: Projects N248799 and N257642
Norwegian Taxonomy Initiative (Artsdatabanken)
Natural Environment Research Council (NERC) | en_US |
dc.description | Source at <a href=https://doi.org/10.1093/aobpla/ply026> https://doi.org/10.1093/aobpla/ply026</a>. | en_US |
dc.identifier.citation | Wang, X., Gusarova, G., Ruhsam, M., de Vere, N., Metherell, C., Hollingsworth, P.M. & Twyford, A.D. (2018). DNA barcoding a taxonomically complex hemiparasitic genus reveals deep divergence between ploidy levels but lack of species-level resolution. <i>AoB Plants, 10</i>(3). https://doi.org/10.1093/aobpla/ply026 | en_US |
dc.identifier.cristinID | FRIDAID 1592626 | |
dc.identifier.doi | 10.1093/aobpla/ply026 | |
dc.identifier.issn | 2041-2851 | |
dc.identifier.uri | https://hdl.handle.net/10037/14727 | |
dc.language.iso | eng | en_US |
dc.publisher | Oxford University Press (OUP) | en_US |
dc.relation.journal | AoB Plants | |
dc.relation.projectID | info:eu-repo/grantAgreement/RCN/UNI-MUSEER/248799/Norway/ForBio - The Research School in Biosystematics// | en_US |
dc.relation.projectID | info:eu-repo/grantAgreement/RCN/FORINFRA/226134/Norway/Norwegian barcode of life network/NorBOL/ | en_US |
dc.relation.projectID | info:eu-repo/grantAgreement/RCN/POLARPROG/257642/Norway/Reindeer interactions from plants and birds to humans: balancing the odds of climate change// | en_US |
dc.rights.accessRights | openAccess | en_US |
dc.subject | VDP::Mathematics and natural science: 400::Zoology and botany: 480 | en_US |
dc.subject | VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 | en_US |
dc.subject | British flora | en_US |
dc.subject | DNA barcoding | en_US |
dc.subject | Euphrasia | en_US |
dc.subject | Orobanchaceae | en_US |
dc.subject | phylogeny | en_US |
dc.subject | polyploidy | en_US |
dc.subject | taxonomic complexity | en_US |
dc.title | DNA barcoding a taxonomically complex hemiparasitic genus reveals deep divergence between ploidy levels but lack of species-level resolution | en_US |
dc.type | Journal article | en_US |
dc.type | Tidsskriftartikkel | en_US |
dc.type | Peer reviewed | en_US |