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dc.contributor.authorHan, Rui
dc.contributor.authorBlencke, Hans-Matti
dc.contributor.authorCheng, Hao
dc.contributor.authorLi, Chun
dc.date.accessioned2019-03-11T12:57:02Z
dc.date.available2019-03-11T12:57:02Z
dc.date.issued2017-12-03
dc.description.abstractPropionibacterium acnes is a commensal bacterium, which is involved in acne inflammation. An antimicrobial peptide named CEN1HC-Br, which was isolated and characterized form the green sea urchin, has been shown to possess broad-spectrum antibacterial activity. Little is known concerning the potential effects of its antibacterial and anti-inflammatory properties against P. acnes. To examine the potency of CEN1HC-Br in acne treatment, we conducted experiments to analyze the antibacterial and anti-inflammatory activities of CEN1HC-Br both in vitro and in vivo. The antimicrobial activity of CEN1HC-Br was evaluated by minimal inhibitory concentration (MIC) assays using the broth dilution method. To elucidate the in vitro anti-inflammatory effect, HaCaT cells and human monocytes were treated with different concentration of CEN1HC-Br after stimulation by P. acnes. The expression of TLR2 and the secretion of the pro-inflammatory cytokines IL-6, IL-8, IL-1β, TNF-α, IL-12, respectively, were measured by enzyme immunoassays. An evaluation of P. acnes-induced ear edema in rat ear was conducted to compare the in vivo antibacterial and anti-inflammatory effect of CEN1HC-Br, the expression of IL-8, TNF-α, MMP-2 and TLR2 was evaluated by immunohistochemistry and real time-PCR. CEN1HC-Br showed stronger antimicrobial activity against P. acnes than clindamycin. CEN1HC-Br significantly reduced the expression of interleukin IL-12p40, IL-6, IL-1β, TNF-α and TLR2 in monocytes, but they were not influenced by clindamycin. Both CEN1HC-Br and Clindamycin attenuated P. acnes-induced ear swelling in rat along with pro-inflammatory cytokines IL-8, TNF-α, MMP-2 and TLR2. Our data demonstrates that CEN1HC-Br is bactericidal against P. acnes and that it has an anti-inflammatory effect on monocytes. The anti-inflammatory effect may partially occur through TLR2 down-regulation, triggering an innate immune response and the inhibition of pro-inflammatory cytokines.en_US
dc.description.sponsorshipNational Natural Science Foundation of China Natural Science Foundation of Zhejiang Province UiT The Arctic University of Norway MABIT research programen_US
dc.descriptionSource at <a href=https://doi.org/10.1016/j.peptides.2017.11.001>https://doi.org/10.1016/j.peptides.2017.11.001</a>.en_US
dc.identifier.citationHan, R., Blencke H.M., Cheng, H. & Li, C. (2018). The antimicrobial effect of CEN1HC-Br against Propionibacterium acnes and its therapeutic and anti-inflammatory effects on acne vulgaris. <i>Peptides, 99</i>, 36-43. https://doi.org/10.1016/j.peptides.2017.11.001en_US
dc.identifier.cristinIDFRIDAID 1530346
dc.identifier.doi10.1016/j.peptides.2017.11.001
dc.identifier.issn0196-9781
dc.identifier.issn1873-5169
dc.identifier.urihttps://hdl.handle.net/10037/14935
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalPeptides
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Dermatology and venereology: 753en_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Dermatologi og venerologi: 753en_US
dc.subjectAntimicrobial peptideen_US
dc.subjectAntiinflammatoryen_US
dc.subjectPropionibacterium acneen_US
dc.subjectAcne vulgarisen_US
dc.titleThe antimicrobial effect of CEN1HC-Br against Propionibacterium acnes and its therapeutic and anti-inflammatory effects on acne vulgarisen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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