Exploring the in vitro expansion of CD4 T cells. For improved culturing of CD4 T cells linked to FNAIT
AuthorHofsten, Susannah von
Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is a rare disease that may cause serious bleedings in the fetus or neonate of a woman who has developed antibodies against the fetus’ platelets. Development of FNAIT has been linked to the presence of platelet reactive CD4 T cells that help B cells to develop into antibody producing plasma cells. To be able to conduct research on such T cells, the Immunology research group must be able to expand and keep them in long term cultures. Recent work revealed that several established T cell clones had started proliferating poorly. In an attempt to understand why and to possibly improve the culturing of future T cell clones, this study looked into some of the conditions that may influence the growth of these cells when expanded in vitro. The established anti- CD3 expansion culture protocol was compared to one that used PHA, but no advantage of using the latter was detected. It was demonstrated that different B-LCLs used as growth promoting feeder cells expressed varying levels of the surface molecules B7-1 and B7-2. This did however not seem to influence their feeder capacity despite the fact that expanding CD4 T cells were shown to express high levels of CD28, which costimulates growth when bound by B7. Expanding CD4 T cells also expressed the inhibitory molecule PD-1, and it was revealed that expression of its ligand, PD-L1, was induced in B-LCLs when used as feeder cells along with PBMCs. Whether this influences the efficiency of an expansion culture is yet to be determined.
PublisherUiT Norges arktiske universitet
UiT The Arctic University of Norway
The following license file are associated with this item: