Drug resistance and the functional role of microRNAs in neuroblastoma
ForfatterBhavsar, Swapnil Parashram
This study is focused on the identification and the functional role of miRNAs in drug resistant neuroblastoma. In paper-I, we performed miRNA-profiling study to elucidate miRNAs that are expressed in the cell lines isolated from the same patients before (parental) and after chemotherapy (resistant). We observed differential expression of 42-miRNAs (34-downregulated and 8-upregulated) across parental and resistant cells. Interestingly, miR-376c-3p and miR-323a-3p were markedly downregulated in resistant cell lines and in a cohort of 226-primary neuroblastoma tumors. In Paper-II and -III, over-expression studies showed that miR-376c-3p and miR-323a-3p targets the oncogenes CCND1 and STAT3, respectively and induce G1-cell cycle arrest and apoptosis in neuroblastoma cells.
Paper I: Roth, S.A., Knutsen, E., Fiskaa, T., Utnes, P., Bhavsar, S.P., Hald, Ø.H. … Einvik, C. (2016). Next generation sequencing of microRNAs from isogenic neuroblastoma cell lines isolated before and after treatment. Cancer Letters, 372(1), 128-136. The paper is available in the file “thesis.pdf”. Published version also available at https://doi.org/10.1016/j.canlet.2015.11.026.
Paper II: Bhavsar, S.P., Løkke, C., Flægstad, T. & Einvik, C. (2018). Hsa-miR-376c-3p targets Cyclin D1 and induces G1-cell cycle arrest in neuroblastoma cells. Oncology Letters, 16(5), 6786-6794. The paper is available in the file “thesis.pdf”. Also available in Munin at https://hdl.handle.net/10037/14622.
Paper III: Bhavsar, S.P., Olsen, L., Løkke, C., Flægstad T. & Einvik, C. Hsa-miR-323a-3p targets STAT3 and induces G1-cell cycle arrest and apoptosis in neuroblastoma cells. (Manuscript). The paper is available in the file “thesis_entire.pdf”.
ForlagUiT The Arctic University of Norway
UiT Norges arktiske universitet
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