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dc.contributor.authorRozenfeld, Christoffer
dc.contributor.authorGarcía-Carpintero, Victor
dc.contributor.authorPérez, Luz
dc.contributor.authorGallego, Victor
dc.contributor.authorHerranz-Jusdado, Juan Germán
dc.contributor.authorTveiten, Helge
dc.contributor.authorJohnsen, Helge K.
dc.contributor.authorFontaine, Romain David
dc.contributor.authorWeltzien, Finn-Arne
dc.contributor.authorCañizares, Joaquín
dc.contributor.authorAsturiano, Juan F.
dc.contributor.authorPenaranda, David Sanchez
dc.date.accessioned2019-09-11T09:25:30Z
dc.date.available2019-09-11T09:25:30Z
dc.date.issued2019-07-22
dc.description.abstract<i>Background</i>: The impossibility of closing the life cycle of the European eel (<i>Anguilla anguilla</i>) in captivity troubles the future of this critically endangered species. In addition, the European eel is a highly valued and demanded resource, thus the successful closing of its life cycle would have a substantial economic and ecological impact. With the aim of obtaining the highest gamete quality, the study of the effects of environmental factors, such as temperature, on reproductive performance may prove valuable. This is especially true for the exposure to cold water, which has been reported to improve sexual development in multiple other <i>Actinopterygii</i> species.<p> <p><i>Results</i>: European eel males treated with cold seawater (10 °C, T10) for 2 weeks showed an increase in the proliferation and differentiation of spermatogonial cells until the differentiated spermatogonial type A cell stage, and elevated testosterone and 11-ketotestosterone plasma levels. Transcriptomes from the tissues of the brain-pituitary-gonad (BPG) axis of T10 samples revealed a differential gene expression profile compared to the other experimental groups, with clustering in a principal component analysis and in heat maps of all differentially expressed genes. Furthermore, a functional analysis of differentially expressed genes revealed enriched gene ontology terms involved in the regulation of circadian rhythm, histone modification, meiotic nuclear division, and others.<p> <p><i>Conclusions</i>: Cold seawater treatment had a clear effect on the activity of the BPG-axis of European eel males. In particular, our cold seawater treatment induces the synchronization and increased proliferation and differentiation of specific spermatogonial cells. In the transcriptomic results, genes related to thermoception were observed. This thermoception may have caused the observed effects through epigenetic mechanisms, since all analysed tissues further revealed differentially expressed genes involved in histone modification. The presented results support our hypothesis that a low temperature seawater treatment induces an early sexual developmental stage in European eels. This hypothesis is logical given that the average temperature experienced by eels in the early stages of their oceanic reproductive migration is highly similar to that of this cold seawater treatment. Further studies are needed to test whether a cold seawater treatment can improve the response of European eels to artificial hormonal treatment, as the results suggest.en_US
dc.description.sponsorshipSpanish Ministry of Science and Innovationen_US
dc.descriptionSource at <a href=https://doi.org/10.1186/s12864-019-5969-6>https://doi.org/10.1186/s12864-019-5969-6. </a> © The Author(s). 2019en_US
dc.identifier.citationRozenfeld, C., García-Carpintero, V., Pérez, L., Gallego, V., Herranz-Jusdado, J.G., Tveiten, H. ... Penaranda, D.S. (2019). Cold seawater induces early sexual developmental stages in the BPG axis of European eel males. <i>BMC Genomics, 20</i>:597. https://doi.org/10.1186/s12864-019-5969-6en_US
dc.identifier.issn1471-2164
dc.identifier.otherFRIDAID 1723191
dc.identifier.other10.1186/s12864-019-5969-6
dc.identifier.urihttps://hdl.handle.net/10037/16158
dc.language.isoengen_US
dc.publisherBMCen_US
dc.relation.journalBMC Genomics
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020-EU.1.3.1./642892/EU/Improved production strategies for endangered freshwater species//en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920en_US
dc.subjectVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920en_US
dc.subjectAnguilla anguillaen_US
dc.subjectRNA-sequencingen_US
dc.subjectEpigeneticsen_US
dc.subjectTemperatureen_US
dc.subjectSpermatogonial proliferationen_US
dc.subjectMigrationen_US
dc.subjectImmunofluorescenceen_US
dc.subjectRadioimmunoassayen_US
dc.subjectHistologyen_US
dc.titleCold seawater induces early sexual developmental stages in the BPG axis of European eel malesen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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