dc.contributor.author | Svenning, Steingrim | |
dc.contributor.author | Gondek-Wyrozemska, Agata T | |
dc.contributor.author | van der Wal, Yorick Andreas | |
dc.contributor.author | Robertsen, Børre | |
dc.contributor.author | Jensen, Ingvill | |
dc.contributor.author | Jørgensen, Jorunn B | |
dc.contributor.author | Edholm, Eva-Stina Isabella | |
dc.date.accessioned | 2019-10-17T07:57:14Z | |
dc.date.available | 2019-10-17T07:57:14Z | |
dc.date.issued | 2019-10-11 | |
dc.description.abstract | Antigen processing and presentation by major histocompatibility complex (MHC) molecules is a cornerstone in vertebrate immunity. Like mammals, teleosts possess both classical MHC class I and multiple families of divergent MHC class I genes. However, while certain mammalian MHC class I-like molecules have proven to be integral in immune regulation against a broad array of pathogens, the biological relevance of the different MHC class I lineages in fish remains elusive. This work focuses on MHC class I L lineage genes and reveals unique regulatory patterns of six genes (<i>Sasa-lia, Sasa-lda, Sasa-lca, Sasa-lga, Sasa-lha</i>, and <i>Sasa-lfa</i>) in antimicrobial immunity of Atlantic salmon (<i>Salmo salar L.</i>). Using two separate <i>in vivo</i> challenge models with different kinetics and immune pathologies combined with <i>in vitro</i> stimulation using viral and bacterial TLR ligands, we show that <i>de novo</i> synthesis of different L lineage genes is distinctly regulated in response to various microbial stimuli. Prior to the onset of classical MHC class I gene expression, <i>lia</i> was rapidly and systemically induced <i>in vivo</i> by the single-stranded (ss) RNA virus salmonid alpha virus 3 (SAV3) but not in response to the intracellular bacterium <i>Piscirickettsia salmonis</i>. In contrast, <i>lga</i> expression was upregulated in response to both viral and bacterial stimuli. A role for distinct MHC class I L-lineage genes in anti-microbial immunity in salmon was further substantiated by a marked upregulation of <i>lia</i> and <i>lga</i> gene expression in response to type I IFNa stimulation <i>in vitro</i>. Comparably, <i>lha</i> showed no transcriptional induction in response to IFNa stimulation but was strongly induced in response to a variety of viral and bacterial TLR ligands. In sharp contrast, <i>lda</i> showed no response to viral or bacterial challenge. Similarly, induction of <i>lca</i>, which is predominantly expressed in primary and secondary lymphoid tissues, was marginal with the exception of a strong and transient upregulation in pancreas following SAV<sub>3</sub> challenge Together, these findings suggest that certain Atlantic salmon MHC class I L lineage genes play important and divergent roles in early anti-microbial response and that their regulation, in response to different activation signals, represents a system for selectively promoting the expression of distinct non-classical MHC class I genes in response to different types of immune challenges. | en_US |
dc.description | Source at <a href=https://doi.org/10.3389/fimmu.2019.02425>https://doi.org/10.3389/fimmu.2019.02425. </a> | en_US |
dc.identifier.citation | Svenning, S., Gondek-Wyrozemska, A.T., van der Wal, Y.A., Robertsen, B., Jensen, I., Jørgensen, J.B. & Edholm, E-S. (2019). Microbial danger signals control transcriptional induction of distinct MHC class I L lineage genes in Atlantic salmon. <i>Frontiers in Immunology, 10</i>:2425. https://doi.org/10.3389/fimmu.2019.02425 | en_US |
dc.identifier.cristinID | FRIDAID 1737445 | |
dc.identifier.doi | 10.3389/fimmu.2019.02425 | |
dc.identifier.issn | 1664-3224 | |
dc.identifier.uri | https://hdl.handle.net/10037/16427 | |
dc.language.iso | eng | en_US |
dc.publisher | Frontiers Media | en_US |
dc.relation.journal | Frontiers in Immunology | |
dc.rights.accessRights | openAccess | en_US |
dc.subject | VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 | en_US |
dc.subject | VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 | en_US |
dc.subject | Atlantic salmon | en_US |
dc.subject | MHC class I | en_US |
dc.subject | L-lineage | en_US |
dc.subject | SAV3 | en_US |
dc.subject | Piscirickettsia salmonis | en_US |
dc.title | Microbial danger signals control transcriptional induction of distinct MHC class I L lineage genes in Atlantic salmon | en_US |
dc.type | Journal article | en_US |
dc.type | Tidsskriftartikkel | en_US |
dc.type | Peer reviewed | en_US |