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dc.contributor.authorGundersen, Mona D.
dc.contributor.authorGoll, Rasmus
dc.contributor.authorFenton, Christopher Graham
dc.contributor.authorAnderssen, Endre
dc.contributor.authorSørbye, Sveinung Wergeland
dc.contributor.authorFlorholmen, Jon
dc.contributor.authorPaulssen, Ruth H
dc.date.accessioned2019-11-07T13:19:58Z
dc.date.available2019-11-07T13:19:58Z
dc.date.issued2019-10-03
dc.description.abstract<i>OBJECTIVES</i>: A healed intestinal mucosa is the aim of therapy in acute ulcerative colitis (UC). Disruption of mucosal wound healing may lead to severe complications including intestinal fibrosis. This study examined mucosal gene expression in the healing process of acute UC with a special focus on known mediators of fibrosis.<p> <p><i>METHODS</i>: Endoscopic biopsies from patients with acute, moderate to severe UC were analyzed with a quantitative polymerase chain reaction array for 84 genes involved in fibrosis pathways. All patients were treated with infliximab (anti– tumor necrosis factor). Biopsies were taken before therapy and when disease remission was reached, defined as a Mayo score of£2, with an endoscopic subscore of 0 or 1. A healthy control group was included. Immunostaining of matrix metallopeptidase 9 and smooth muscle actin was performed.<p> <p><i>RESULTS</i>: Mucosal biopsies from acute UC (n 5 28), remission UC (n 5 28), and healthy controls (n 5 13) were analyzed. Fibrosis and extracellular matrix-associated genes were upregulated in the endoscopically healed UC mucosa vs controls, with collagen type III alpha 1 chain, actin alpha 2, lysyl oxidase, TIMP metallopeptidase inhibitor 3, and caveolin 1 uniquely showing no overlap with acute disease. Pro- and antifibrotic mediators (interleukin [IL]13 receptor subunit alpha 2, IL1B, IL10, tumor necrosis factor, snail family transcriptional repressor 1, and C-C motif chemokine ligand 2) were upregulated in both acute and healed UC compared with controls. An attenuated pattern of the canonical transforming growth factor beta (TGFB) pathway was observed in acute UC and to a lesser extent in the healed mucosa, except for TGFB2, which was enhanced.<p> <i>DISCUSSION</i>: The endoscopically healed mucosa of UC showed a persisting dysregulation of fibrosis-associated mediators compared with controls, including extracellular matrix remodeling, profibrotic cytokines, and TGFB signaling pathways.en_US
dc.descriptionSource at <a href=https://doi.org/10.14309/ctg.0000000000000082>https://doi.org/10.14309/ctg.0000000000000082. </a> © 2019 The Author(s).en_US
dc.identifier.citationGundersen, M.D., Goll, R., Fenton, C.G., Anderssen, E., Sørbye, S.W., Florholmen, J.R. & Paulssen, R.H. (2019). Fibrosis Mediators in the Colonic Mucosa of Acute and Healed Ulcerative Colitis. <i>Clinical and Translational Gastroenterology, 10</i>(10): e00082. https://doi.org/10.14309/ctg.0000000000000082en_US
dc.identifier.cristinIDFRIDAID 1744595
dc.identifier.doi10.14309/ctg.0000000000000082
dc.identifier.issn2155-384X
dc.identifier.urihttps://hdl.handle.net/10037/16620
dc.language.isoengen_US
dc.publisherLippincott, Williams & Wilkinsen_US
dc.relation.journalClinical and Translational Gastroenterology
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleFibrosis Mediators in the Colonic Mucosa of Acute and Healed Ulcerative Colitisen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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